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EC number: 955-780-3 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Justification for type of information:
- Experimental test result performed using standard OECD test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- GLP compliance:
- no
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 0 (control), 100 mg/l
- Sampling method: The test solution was prepared by dissolving 400 mg of test chemical in 4000 ml of RO water to get final concentration of 100 mg/l. The same stock solution was used as test concentration. - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
-Method: The test solution was prepared by dissolving 400 mg of test chemical in 4000 ml of RO water to get final concentration of 100 mg/l. The same stock solution was used as test concentration. - Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name: Zebra fish
- Source: Anu Pet Shop Nagpur
- Length at study initiation (length definition, mean, range and SD): 1.9 cm
- Weight at study initiation (mean and range, SD): 0.15 g
ACCLIMATION
- Acclimation period: 7 days
- Feeding frequency during acclimation: Daily once
- Type and amount of food: Food was provided during acclimatization.
- Health during acclimation (any mortality observed): No
- Aeration: Continuous - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Hardness:
- 100 mg of CaCO3
- Test temperature:
- 21 to 25 °C
- pH:
- 7.7
- Dissolved oxygen:
- 86.83 %
- Salinity:
- Not applicable
- Nominal and measured concentrations:
- Nominal concentrations: 0 (control), 100 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Glass aquaria having 7 lit capacity
- Type (delete if not applicable): open
- Total volume of solution: 4 L
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): Replicate
- No. of vessels per control (replicates): Replicate
- No. of vessels per vehicle control (replicates): Not applicable
- Biomass loading rate: 7 fishes/ 4 L of test solution
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: RO water
OTHER TEST CONDITIONS
- Photoperiod: 16 hr light and 8 hr darkness
- Light Intensity : 821.25 lux
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mortality
VEHICLE CONTROL PERFORMED: Not applicable
RANGE-FINDING STUDY
Based on the available data on the test chemical range finding test was not conducted directly main study was intaited to draw the conclusion on test substance toxicity. - Reference substance (positive control):
- not required
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: Other details not known
- Details on results:
- - Other abnormalities: No abnormal symptoms in control and test vessel
- Mortality of control: No mortality
- Other adverse effects control: No adverse effects - Sublethal observations / clinical signs:
Table 1 : Result on Mortality and Visible Symptoms
Sr. No
Test concentrations
(mg/ L)
3 hr
6 hr
24 hr
48 hr
72 hr
96 hr
Cumulative Mortality
Percent Inhibition
1
control
-
-
-
-
-
-
-
-
2
100
-
-
-
-
-
-
-
-
Table 2 : Result on Mortality and Visible Symptoms
Sr. No
Test concentrations (mg/ L)
3 hr
6 hr
24 hr
48 hr
72 hr
96 hr
1
control
NS 7
NS 7
NS 7
NS 7
NS 7
NS 7
2
100
NS 7
NS 7
NS 7
NS 7
NS 7
NS 7
Any other symptoms
-
-
-
-
-
-
EXPLANATION OF SYMPTOMS:
The number behind symbol for symptom = number of affected fish
A: apathy, NR : Narcotic-like state, C: Swimming near water surface, M: Slow movements compared to control, NS: No abnormal symptoms, SC: Schooling effect , L : Loss of equilibrium, SM: Shimming movement, H 1 : Hyperactivity, H 2 : Hypoactivity B : Bending behavior, F : Fin rot ,IS: Invert swimming, SB: Swimming behavior, RF: Respiratory Function, P: Pigmentation, E: Exophthalmos
Table 3: Result on pH
Test Concentrations (mg/L)
p H
control
7.9
7.5
7.5
7.7
100
7.8
7.9
7.8
8.1
Table 4: Result on temperature
Test concentrations (mg /L)
Temperature °C
24 Hr
48 Hr
72 Hr
96 Hr
control
23.3
23.1
23.5
22.4
100
23.3
23.3
23.5
22.4
Table 5: Result on Dissolved oxygen
Test Concentrations ( mg/L)
Dissolved Oxygen
24 Hr
48 Hr
72 Hr
96 Hr
control
80.7
75.0
68.0
70
100
74.7
69.4
64.6
86.7
- Validity criteria fulfilled:
- yes
- Remarks:
- No mortality was found in the control at end of test. The DO concentration remained above 60% of air- saturation value throughout, exposure period (reported as 86.83%).Test concentrations were measured, found to remain at 80-120% of nomina concentration.
- Conclusions:
- Based on mortality effect of test chemical on test organism Danio rerio (Zebra fish) the 96 hr LC50 value was determined to be > 100 mg/l.
- Executive summary:
The study was conducted in accordance with OECD Guideline 203, Fish, Acute Toxicity Test ( 2019 ). A study was performed to assess the Acute toxicity of test chemical, to Danio rerio (Zebra fish) under static conditions. Source of test organisms was Anu Pet Shop Nagpur. Length at study initiation 1.9 cm (avg). Weight at study initiation was 0.15 g (avg). Test organism was acclimatized for a period of 7 days. Feeding frequency during acclimation once in a day. Food was provided during acclimatization. No mortality observed was observed during acclimatization. Continuous aeration was also provided during acclimatization. The test solution was prepared by dissolving 400 mg of test chemical in 4000 ml of RO water to get final concentration of 100 mg/l. The same stock solution was used as test concentration. The main study (i.e. limit test) was conducted using 0 (control) and 100 mg/L test concentration. Test vessel was taken as Glass aquaria having 7 lit capacity. Type of test vessel was of open type. Total volume of solution was 4 L. Number of organisms per vessel was taken as seven. Number of vessel per concentration was taken in replicate. The number of vessels per control was taken in the replicate. The biomass loading rate was of 7 fishes/ 4 L of test solution. The analytical determinations were performed by UV- VIS spectrophotometer. Analytical assessments were performed for selected test concentrations at 0 Hr and 96 Hr. Environmental parameters such as pH (7.7), temperature (21 to 25 °C), dissolved oxygen (86.83 %), hardness (100 mg of CaCO3), photoperiod (16 h light- 8 h dark) with light intensity of 821.25 lux was maintained in acceptable range throughout the test. Thus, fulfilling the validity criteria includes, no mortality was found in the control by the end of the test. The dissolved oxygen concentration remained above 60 % of the air- saturation value throughout, exposure period. And the test concentrations were measured and found to remain at 120-80 % of nominal concentration. Based on mortality effect of test chemical on test organism Danio rerio (Zebra fish) the 96 hr LC50 value was determined to be > 100 mg/l. As per value of LC50 the test chemical was considered to be ‘not classified' as per the CLP classification criteria Thus, considered as non-toxic to fishes.
Reference
Description of key information
Based on mortality effect of test chemical on test organism Danio rerio (Zebra fish) the 96 hr LC50 value was determined to be > 100 mg/l.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- > 100 mg/L
Additional information
Various experimental studies of test chemical and its read across chemical were reviewed for short term toxicity to fishes endpoint which are summarised as below:
In an experimental study from study report (2022) for the target chemical, the study was conducted in accordance with OECD Guideline 203, Fish, Acute Toxicity Test (2019). A study was performed to assess the Acute toxicity of test chemical, to Danio rerio (Zebra fish) under static conditions. Source of test organisms was Anu Pet Shop Nagpur. Length at study initiation 1.9 cm (avg). Weight at study initiation was 0.15 g (avg). Test organism was acclimatized for a period of 7 days. Feeding frequency during acclimation once in a day. Food was provided during acclimatization. No mortality observed was observed during acclimatization. Continuous aeration was also provided during acclimatization. The test solution was prepared by dissolving 400 mg of test chemical in 4000 ml of RO water to get final concentration of 100 mg/l. The same stock solution was used as test concentration. The main study (i.e. limit test) was conducted using 0 (control) and 100 mg/L test concentration. Test vessel was taken as Glass aquaria having 7 lit capacity. Type of test vessel was of open type. Total volume of solution was 4 L. Number of organisms per vessel was taken as seven. Number of vessel per concentration was taken in replicate. The number of vessels per control was taken in the replicate. The biomass loading rate was of 7 fishes/ 4 L of test solution. The analytical determinations were performed by UV- VIS spectrophotometer. Analytical assessments were performed for selected test concentrations at 0 Hr and 96 Hr. Environmental parameters such as pH (7.7), temperature (21 to 25 °C), dissolved oxygen (86.83 %), hardness (100 mg of CaCO3), photoperiod (16 h light- 8 h dark) with light intensity of 821.25 lux was maintained in acceptable range throughout the test. Thus, fulfilling the validity criteria includes, no mortality was found in the control by the end of the test. The dissolved oxygen concentration remained above 60 % of the air- saturation value throughout, exposure period. And the test concentrations were measured and found to remain at 120-80 % of nominal concentration. Based on mortality effect of test chemical on test organism Danio rerio (Zebra fish) the 96 hr LC50 value was determined to be > 100 mg/l. As per value of LC50 the test chemical was considered to be ‘not classified' as per the CLP classification criteria Thus, considered as non-toxic to fishes.
Another acute study to fish has been carried for assessing the toxic effect of test chemical. The study was conducted by following the OECD Guideline 203 (Fish, Acute Toxicity Test) in a static system for a period of 96 days. Brachydanio rerio (Zebra fish) was used as a test organism.Test chemical concentrations were verified analytically. On the basis of the effect of the test chemical on mortality of test organism Brachydanio rerio (Zebra fish), the 96 hr LC0 and LC50 value was determined to be 500 and >500 mg/l (nominal conc.), respectively. Thus on the basis of these values, test chemical was considered as non-toxic to aquatic fish and hence, considered to be ‘’not classified’’ as per the CLP classification criteria.
Also, another acute toxicity study was conducted for 96 hrs for assessing the effect of test chemical on fish. The test was performed following the OECD guideline No. 203 “Fish Acute Toxicity Test”. Zebra fish (Danio rerio) of average weight 0.066 g and average length of 1.7 cm was used as a test organism for the study. Test fishes were kept in a static tank in tap water passed through reverse osmosis system, under natural conditions along with proper feed and aeration. During the housing period, test fishes were fed once daily with standard brand fed. The test conditions during the housing of the test organisms were oxygen content of 7.0 mg/l, pH 7.4, water temperature 24°C and under a photoperiod of 16:8 hr light: dark conditions, respectively. Test concentrations selected for the study was were 0, 6.25, 12.5, 25, 50 and 100 mg/L, respectively. Total 8 fishes were exposed to test chemical in a 5 lit bowl aquaria containing 4 liters of potable water. The test vessels were placed in a room at a temperature of 24°C, pH 7.2, hardness of water 150.5 mg of CaCO3 and under a photoperiod of 16:8 hr light: dark conditions, respectively. Aeration in test vessels was provided 1 day before the start of the experiment. No mortalities were observed in the control and test vessel. On the basis of effect of test chemical on mortality of the test organism, the median lethal concentration (LC50 (96 h)) value was determined to be > 100 mg/l. Thus, test chemical was considered as non-toxic to aquatic fishes and hence, considered to be not classified as per the CLP classification criteria.
On the basis of the above-mentioned information, the test chemical was considered to be 'not classified' as per the CLP classification criteria. Thus, test chemical was considered as non-toxic to fishes.
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