Reaction products of diazotised sodium 2-amino-5-{[2-(sulfonatooxy)ethyl]sulfonyl}benzenesulfonate coupled with sodium 2-(4-aminobenzene-1-sulfonyl)ethyl sulfate, subsequently coupled with diazotised sodium 2-(4-aminobenzene-1-sulfonyl)ethyl sulfate

Water solubility

Link to relevant study record(s)

Reference

Endpoint:

water solubility

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

Experimental test results performed using standard test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 105 (Water Solubility)

GLP compliance:

no

Type of method:

flask method

Key result

Water solubility:

164.852 g/L

Temp.:

25 °C

Remarks on result:

other: other details are not known

OBSERVATIONS:

For standard:

Sr. No	Concentrations of standard solutions (mg/L)	Absorbance	Aliquot of stock solution taken (µL)	Amount of water added to make up the volume (mL)
1	Blank (water)	0.0001	-	-
2	26.5320	0.6176	4000	6.0
3	33.1650	0.8378	5000	5.0
4	39.7980	1.0048	6000	4.0
5	46.4310	1.1369	7000	3.0
6	53.0640	1.3218	8000	2.0
7	59.6970	1.4923	9000	1.0

The standard curve was plotted between absorbance versus concentrations (mg/L).

 

CONFIRMATORY TEST AT 24, 48 AND 72 h

Sample stirring (h)	Concentration obtained		Average	Absorbance	Average	Dilution factor	Water Solubility value obtained (mg/L)	Average
24 h	R1	30.7609	30.7592	0.7648	0.7648	5000	153804.5000	153795.7500
	R2	30.7574		0.7647			153787.0000
48 h	R1	34.6204	34.6193	0.8603	0.8603	5000	173102.0000	173096.2500
	R2	34.6181		0.8603			173090.5000
72 h	R1	33.3735	33.3708	0.8293	0.8293	5000	166867.5000	166854.0000
	R2	33.3681		0.8292			166840.5000

CALCULATION

The water solubility for 24 h, 48 h and 72 h was calculated as follows: Mean water solubility value for 24 h + 48 h + 72 h run sample.

= (153795.7500 + 173096.2500 + 166854.0000)/3

= 164.582 g/L

Conclusions:

The mean water solubility value of test chemical was determined to be 164.582 g/L with a relative standard deviation of ±5.98 at 25±2°C.

Executive summary:

The study was conducted to determine the solubility of test chemical in ultra-pure water, according to the OECD guideline 105 (Adopted by the Council on 27th July 1995) and OECD 101(Adopted: 12 May 1981). Test was conducted at 25±2 °C in two stages; preliminary test , followed by confirmatory test for 24 h, 48 h and 72 h in replicates. The final water solubility value was calculated based on average of the individual values obtained after 24 h, 48 h and 72 h. The difference between the average values in three vessels was not observed more than 15%. The water solubility value was determined to be 164.582 g/L with a relative standard deviation of ±5.98 at 25±2 °C.Thus it can be concluded that the test chemical is highly soluble in water . 

 

Description of key information

The mean water solubility value of test chemical was determined to be 164.582 g/L with a relative standard deviation of ±5.98 at 25±2°C.

Key value for chemical safety assessment

Water solubility:

164.58 g/L

at the temperature of:

25 °C

Additional information

The study was conducted to determine the solubility of test chemical in ultra-pure water, according to the OECD guideline 105 (Adopted by the Council on 27th July 1995) and OECD 101(Adopted: 12 May 1981). Test was conducted at 25±2 °C in two stages; preliminary test , followed by confirmatory test for 24 h, 48 h and 72 h in replicates. The final water solubility value was calculated based on average of the individual values obtained after 24 h, 48 h and 72 h. The difference between the average values in three vessels was not observed more than 15%. The water solubility value was determined to be 164.582 g/L with a relative standard deviation of ±5.98 at 25±2 °C.Thus it can be concluded that the test chemical is highly soluble in water .