Reaction products of diazotised sodium 2-amino-5-{[2-(sulfonatooxy)ethyl]sulfonyl}benzenesulfonate coupled with sodium 2-(4-aminobenzene-1-sulfonyl)ethyl sulfate, subsequently coupled with diazotised sodium 2-(4-aminobenzene-1-sulfonyl)ethyl sulfate

Endpoint summary

Description of key information

Appearance / physical state / colour 

On the basis of physical observation, test chemical was observed to be reddish brown fine powder which appears as dark orange solution in aqueous medium 

 

Melting point 

The test chemical does not melt, but decomposes with colour change from dark brown to black. Thus, mean decomposition temperature of test chemical was determined to be 252.50 °C at 958.2 hPa .

 

Boiling point 

the study does not need to be conducted because chemical change occurred during the melting point study

 

Density

The mean pour density and tapped density of test chemical was determined to be 0.616 g/cm3 and 0.730 g/cm3 at 20 °C and 962.4 hPa.

 

Particle size distribution (Granulometry)

The particle size distribution was determined as percentage distribution of test chemical in various sieves. the particle size distribution was determined to be in the range of 150 micron to 53 micron.

 

Vapour pressure

the test chemical decomposes before melting point is reached , therefore the study cannot be performed .

 

Partition coefficient 

The Partition coefficient (Pow) of the test chemical was determined to be -2.812±0.229 at 25 °C± 1°C.

 

Water solubility

The mean water solubility value of test chemical was determined to be 164.582 g/L with a relative standard deviation of ±5.98 at 25±2°C.

 

Surface tension 

the study does not need to be conducted because surface activity is not a desired property of the material

 

Flash point

the study does not need to be conducted because the flash point is only relevant to liquids and low melting point solids

 

Auto flammability 

Test chemical did not catch fire on being exposed to air at room temperature of 27°C pressure of 962 hPa. This indicates that the test chemical is not auto-flammable.

 

Flammability 

Test chemical does not indicate any ignition when the flame of bunsen burner having temperature of approximately 950°C is brought in contact with it. Thus it can be concluded that test chemical is not flammable.

 

Explosiveness

the study does not need to be conducted because there are no chemical groups present in the molecule which are associated with explosive properties

 

pH

The experimental pH value for 1% aqueous solution of test chemical  was determined to be 4.76 at 24.4°C ,inferring its  acidic nature.

Additional information

Appearance / physical state / colour 

On the basis of physical observation, test chemical was observed to be reddish brown fine powder which appears as dark orange solution in aqueous medium 

 

Melting point 

A study was performed to determine the melting point of test chemical in atmospheric pressure.

The study was conducted in accordance with OECD 102 Guideline for testing of chemicals, for Melting Point Adopted by Council on 27^th July1995.

The test chemical does not melt, but decomposes with colour change from dark brown to black. Thus, mean decomposition temperature of test chemical was determined to be  252.50 °C at 958.2 hPa .

 

Boiling point 

the study does not need to be conducted because chemical change occurred during the melting point study

 

Density

A study was performed to determine the density of test chemical in respective atmospheric pressure and temperature conditions.

The study was conducted in accordance with OECD guideline for the Testing of Chemicals no. 109; Density of Liquid and Solids, Adopted: 2 October 2012 and Adopted from method recommended in the International Pharmacopoeia, fourth Edition, 2014

The mean pour density and tapped density of test chemical was determined to be 0.616 g/cm³ and 0.730 g/cm³ at 20 °C and 962.4 hPa.

 

Particle size distribution (Granulometry)

A study was performed to determine the particle size distribution of test chemical within range of 150 µ to 10 µ. The study was conducted in accordance with OECD guideline 110 and according to method adapted from ASTM B214-99.” The particle size distribution was determined to be in the range of  150 micron to 53 micron.

 

Vapour pressure

the test chemical decomposes before melting point is reached , therefore the study cannot be performed .

 

Partition coefficient 

The partition Coefficient n-octanol/water of test chemical was determined by OECD Guideline No. 107, adopted on 27th July 1995,”Shake Flask Method”.

Two solvent were mutually pre-saturated at experiment temperature 25°C± 1°C. on the mechanical shaker, one containing n-Octanol with 0.280 mL of water and the other containing water with 0.470 mL of n-octanol for 100mL stock were shaken for 24 hours on a mechanical shaker. A stock solution was be prepared in n-Octanol (presaturated with water) by accurately weighing the test chemical which was introduced into 30 mL (1:1), 20 mL (1:2) and 40 mL (2:1) of pre-saturated n-Octanol respectively and mix with ratios of pre-saturated water. After separation, both the aqueous phase and n-Octanol phase were analyzed by Spectrophotometer.

 The partition coefficient was calculated, from the measured concentration of test chemical in the organic and aqueous phases. The log Pow value was determined to be -2.812±0.229 at 25°C± 1°C.Thus it can be concluded that the test chemical is hydrophilic in nature . 

 

 

Water solubility

The study was conducted to determine the solubility of test chemical in ultra-pure water, according to the OECD guideline 105 (Adopted by the Council on 27th July 1995) and OECD 101(Adopted: 12 May 1981). Test was conducted at 25±2 °C in two stages; preliminary test , followed by confirmatory test for 24 h, 48 h and 72 h in replicates. The final water solubility value was calculated based on average of the individual values obtained after 24 h, 48 h and 72 h. The difference between the average values in three vessels was not observed more than 15%. The water solubility value was determined to be 164.582 g/L with a relative standard deviation of ±5.98 at 25±2 °C.Thus it can be concluded that the test chemical is highly soluble in water . 

 

Surface tension 

the study does not need to be conducted because surface activity is not a desired property of the material

 

Flash point 

the study does not need to be conducted because the flash point is only relevant to liquids and low melting point solids

 

Auto flammability 

Test chemical did not catch fire on being exposed to air at room temperature of 27°C pressure of 962 hPa. This indicates that the test chemical is not auto-flammable.

 

Flammability 

Test chemical does not indicate any ignition when the flame of bunsen burner having temperature of approximately 950°C is brought in contact with it. Thus it can be concluded that test chemical is not flammable.

 

Explosiveness

the study does not need to be conducted because there are no chemical groups present in the molecule which are associated with explosive properties

 

pH

A study was performed to determine the pH of test chemical at respective temperature.

The study was conducted in accordance with OECD 122, Guideline for testing of chemicals, for pH Adopted by Council on 26^th July, 2013.

pH value for 1% aqueous solution of test chemical was determined to be 4.76 at 24.4 °C ,inferring its  acidic nature.