3,7-dimethylnon-6-en-1-yn-3-ol

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Remarks:

Type of genotoxicity: gene mutation

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: well documented guideline study

Cross-reference

Cross-reference 1

Reason / purpose for cross-reference:

reference to same study

Cross-reference 2

Reason / purpose for cross-reference:

reference to other study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1996

Report date:

1996

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 471 (Bacterial Reverse Mutation Assay)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

3,7-dimethylnon-6-en-1-yn-3-ol

EC Number:

246-061-9

EC Name:

3,7-dimethylnon-6-en-1-yn-3-ol

Cas Number:

24173-47-5

Molecular formula:

C11H18O

IUPAC Name:

3,7-dimethylnon-6-en-1-yn-3-ol

Method

Target gene:

TA97a: hisD6610, TA 98: hisD3052; TA 100 and TA 1535 hisG46, TA102: hisG428

Species / strain

Species / strain / cell type:

S. typhimurium, other: TA97. TA98, TA100, TA102, TA1535

Additional strain / cell type characteristics:

other: rfa, delta-uvrB, pKM101, pAQ1

Metabolic activation:

with and without

Metabolic activation system:

S9

Test concentrations with justification for top dose:

0, 1.6, 5, 16.6, 50, 166, 500

Controls

Untreated negative controls:

yes

Negative solvent / vehicle controls:

yes

Positive controls:

yes

Positive control substance:

2-nitrofluorene

sodium azide

mitomycin C

other: 2-Aminoanthracene

Results and discussion

Test results

Species / strain:

other: TA97. TA98, TA100, TA102, TA1535

Metabolic activation:

with and without

Genotoxicity:

negative

Untreated negative controls validity:

valid

Positive controls validity:

valid

Remarks on result:

other: all strains/cell types tested

Remarks:

Migrated from field 'Test system'.

Any other information on results incl. tables

-

Applicant's summary and conclusion

Conclusions:

Interpretation of results (migrated information):
negative

It is concluded that neither Ethyldehydrolinalool per se, nor
any of the metabolites formed under the described experimental conditions is
mutagenic in the Ames test.

Executive summary:

Ethyldehydrolinalool was evaluated for mutagenic activity in the Ames test. A standard plate incorporation and a preincubation modification assay in absence and in presence of an exogenous metabolic activation system (S9) were

performed. Five Salmonella typhimurium tester strains (TA1535, TA97, TA98, TA100, and TA102) were employed. The activity of the S9-mix and the responsiveness of the tester strains were verified by including appropriate controls into each experiment.

Ethyldehydrolinalool was dissolved in dimethylsulfoxide (DMSO). Strong toxic effects were observed in a preliminary toxicity experiment starting at 1’666 ug/plate (no growth). Therefore concentrations ranging from 10 to 1’000 ug/plate were

evaluated in the main experiments. No precipitation of the compound was observable

up to the highest test concentration of 1’000 ug/plate using the standard procedure,

whereas a slighty turbidity at the top dose was observed with the preincubation modification assay. Strain dependent toxic effects were observed (reduction in the number of-revertants and/or reduction in the background growth). Toxicity was more

pronounced using the preincubation method, known to be more sensitive for several class-of-compounds.

No increase of the number of mutant colonies was apparent in all five investigated tester strains.