Phosphonic acid, P-2-propen-1-yl-, diethyl ester

Eye irritation

Administrative data

Endpoint:

eye irritation: in vitro / ex vivo

Type of information:

experimental study

Adequacy of study:

key study

Study period:

28 Jan 2015

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: study according to the guideline under GLP. Study does not allow definitive conclusion

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2015

Report date:

2015

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 437 (Bovine Corneal Opacity and Permeability Test Method for Identifying Ocular Corrosives and Severe Irritants)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Diethyl allylphosphonate

EC Number:

619-646-5

Cas Number:

1067-87-4

Molecular formula:

C7H15O3P

IUPAC Name:

Diethyl allylphosphonate

Test material form:

other: liquid

Details on test material:

Identification: Diethyl Allylphosphonate
Appearance/Physical State: Clear colorless liquid
Storage Conditions: Room temperature in the dark

Test system

Vehicle:

other: 0.9% w/v sodium chloride solution

Amount / concentration applied:

Test item: as such
Positive control: ethanol > 98.8%
Negative control:0.9% w/v sodium chloride.

Duration of treatment / exposure:

10 min

Details on study design:

Bovine corneas were obtained from a local abatoir, preserved in HBBS and prepared on arrival in the laboratory. Corneas free of damage were mounted in a closed BCOP holder. Both chambers were filled with MEM and incubated for 60 minutes at 32 ± 1 ºC.
In the test undamaged corneas (3/treatment) were checked for opacity (zero measurement) and exposed to 0.75 mL the test substance, negative or positive control (anterior chamber) at 32 ± 1 ºC for 10 minutes. After exposure the chambers and corneas were rinsed and chambers were filled with fresh MEM. Corneas were checked visually and opacity was assessed after incubation for 120 min at 32 ± 1 ºC. In additon the corneas were exposed to sodium fluorin (4 mg/L) (anterior chamber) for 90 minutes. Optical density at 492 nm of the medium in the posterior chamber was measured to assess permeability.
Measurements for opacity were corrected for the initial opacity and the opacity measured in the vehicle controls.
Measurements for permeability were corrected for vehicle control.
All results were averaged.

Results and discussion

In vivo

Results

Irritation parameter:

other: IVIS

Score:

25.4

Remarks on result:

other: mean opacity value + (15 x mean OD492 value)

Any other information on results incl. tables

	Opacity Post incubation – pre-treatment	Opacity corrected	Permeability	Permeability corrected	IVIS
Neg control	1		0.072
	2		0.043
	1		0.058
	Mean 1.3		Mean 0.058		2.2
Pos control	30	28.7	1.495	1.437
	27	25.7	1.490	1.432
	24	22.7	1.298	1.240
		Mean 25.7		Mean 1.370	46.2
Test substance	21	19.7	0.398	0.340
	15	13.7	0.385	0.327
	25	23.7	0.671	0.613
		Mean 19.0		Mean 0.427	25.4

 

Applicant's summary and conclusion

Interpretation of results:

other: no prediction can be made

Remarks:

Criteria used for interpretation of results: EU

Conclusions:

The outcome of the BCOP test with the substance shows that no prediction on the eye irritation of the substance can be made.

Executive summary:

The substance is tested in the in vitro BCOP assay. The calculated IVIS based on assessments of opacity and permeability for the test substance is 6.1. This does not allow a definitive conclusion on the irritant properties of the test substance, but indicates that the substance is not causing serious eye damage.