Benzenepropanenitrile, 2-chloro-.beta.-oxo-

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Study period:

29 August - 19 September 2000

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: The study was conducted in GLP compliance and in accordance with several internationally achnowledged guidelines for the testing of chemicals.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2000

Report date:

2000

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 423 (Acute Oral toxicity - Acute Toxic Class Method)

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method B.1 tris (Acute Oral Toxicity - Acute Toxic Class Method)

Deviations:

no

Test guideline 3

Qualifier:

according to guideline

Guideline:

EPA OPPTS 870.1100 (Acute Oral Toxicity)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test type:

acute toxic class method

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Chlorobenzoylacetonitril

IUPAC Name:

Chlorobenzoylacetonitril

Test material form:

other: solid

Details on test material:

Name: Chlorobenzoylacetoniril
Batch No. 46
Physical state: solid
Colour: yellow
Molecular weight: 179.61 g/mol
Purity: > 99%
Storage: in original container at 4°C

Test animals

Species:

rat

Strain:

Wistar

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Harlan Winkelmann GmbH, Borchen
- Weight at study initiation: male: 174-226g, female: 174-189g.
- Housing: kept in Matrolon cages on Altromin saw fiber beddings
- Diet (e.g. ad libitum): ad libitum diets for rats and rabbits
- Water (e.g. ad libitum): tap water ad libitum
- Acclimation period: adequate
- Fasting: overnight prior to start of test

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22°C +/- 3°C
- Humidity (%): 55% +/- 10%
- Air changes (per hr): 10
- Photoperiod (hrs dark / hrs light): 12:12 hrs

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

DMSO

Details on oral exposure:

VEHICLE
- Concentration in vehicle: 2000 mg test item were mixed in DMSO ad 4 mL.
- Amount of vehicle (if gavage): 4mL
- Justification for choice of vehicle: non-toxic characteristics of vehicle
- Lot/batch no.: 401105/1 32499



MAXIMUM DOSE VOLUME APPLIED: 2000 mg/kg bodyweight

Doses:

single dose
- Concentration in vehicle: 2000 mg test item were mixed in DMSO ad 4 mL.
- Amount of vehicle (if gavage): 4mL
- Justification for choice of vehicle: non-toxic characteristics of vehicle
- Lot/batch no.: 401105/1 32499



MAXIMUM DOSE VOLUME APPLIED: 2000 mg/kg bodyweight

No. of animals per sex per dose:

3 per sex

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: prior to first application and once a week after
- Necropsy of survivors performed: yes (gross pathology)
- Other examinations performed: cage side observations were made on a daily base

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

> 2 000 mg/kg bw

Mortality:

none

Clinical signs:

other: Some clinical signs of toxicity were observed throughout the observation period. Beside a reduced spontaneous activity for the first five days, all animals showed tiredness up to lethargy for the first two days after application.

Other findings:

None

Applicant's summary and conclusion

Interpretation of results:

practically nontoxic

Remarks:

Migrated information Criteria used for interpretation of results: EU

Conclusions:

The acute oral toxicity LD50 of the test substance in rats was determined to be > 2000 mg/kg bw.