Benzenepropanenitrile, 2-chloro-.beta.-oxo-

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2000

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: The study was conducted in GLP compliance and in accordance with several internationally acknowledged guidelines for the testing of chemicals.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2000

Report date:

2000

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test)

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)

Deviations:

no

Test guideline 3

Qualifier:

according to guideline

Guideline:

EPA OPPTS 835.3110 (Ready Biodegradability)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Chlorobenzoylacetonitril

IUPAC Name:

Chlorobenzoylacetonitril

Test material form:

other: solid

Details on test material:

- Name of test material (as cited in study report): Chlorobenzoylacetonitril
- Physical state: solid
- Analytical purity: > 99%
- Purity test date: 22 Dec 1999
- Lot/batch No.: 46
- Storage condition of test material: stored at 4°C in original container

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic (adaptation not specified)

Details on inoculum:

- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): activated sludge from a domestic sewage treatment plant, Groß-Zimmern, Germany
- Preparation of inoculum for exposure: Washing with tap water, centrifugation, decantation of supernatant (twice). Drying of sludge susension, weighing, mixing with test water, aeration until use.
- Concentration of sludge: 1.5g dry material/L.

Duration of test (contact time):

28 d

Initial test substance concentration

Initial test substance concentration 1

Initial conc.:

25.1 other: mg in 244mL

Based on:

test mat.

Initial test substance concentration 2

Initial conc.:

25.6 other: mg in 244 mL

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

TEST CONDITIONS
- Composition of medium: analytical grade salts suspended in deionized water
- Test temperature: 22°C
- pH: 7.6 at test start
- pH adjusted: no
- Continuous darkness: yes
- suspended solids: 30mg/L


TEST SYSTEM
- Culturing apparatus: 500mL manometric flasks
- Number of culture flasks/concentration: 2
- Method used to create aerobic conditions: aeration of sludge prior to use, headspace in flasks filled with air.
- Measuring equipment:
- Test performed in closed vessels due to significant volatility of test substance: flasks were closed


CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Abiotic sterile control: yes
- Toxicity control: yes, aniline

Reference substance

Reference substance:

aniline

Results and discussion

% Degradation

Parameter:

other: ThODNH4

Value:

> 9 - < 12

Sampling time:

28 d

BOD5 / COD results

Results with reference substance:

Aniline passed the ready biodegradability level of 60% within 5 days. The study is therefore considered valid.

Any other information on results incl. tables

The abiotic control did not induce any oxygen consumption.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

other: not readily biodegradable

Conclusions:

The test substance was determined to be not readily biodegradable.