Phosphinic acid, P-[3-(acetyloxy)-3-cyanopropyl]-P-methyl-, butyl ester

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Endpoint:

toxicity to microorganisms

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From Dec 1992 to Dec 11 1992

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study without detailed documentation

Qualifier:

according to guideline

Guideline:

DIN 38412-8 (Pseudomonas Zellvermehrungshemmtest)

GLP compliance:

yes

Remarks:

The Report was drawn in a simplified form. No inspection by the Quality Assurance Unit took place. There is thus no Quality Assurance Statement (test procedure according to GLP) included in this report.

Analytical monitoring:

not specified

Vehicle:

not specified

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: Preparation of stock solution:
1.25 g/L test material stirred for 18 h at 23-24 °C. The pH after stirring (3.8) was corrected to 7.1 with NaOH, 1 mol/l; HCL, 0.1 mol/l
The test material was clearly dissolved. The batch was used as stock solution.

Test organisms (species):

Pseudomonas putida

Details on inoculum:

The strain Pseudomonas putida Migula, DSM 50026 was used as inoculum. The pre-culture and inoculation of the test batch were carried out as stated in the cited DIN guideline (Section 11.2). Prior to the test, the bacterial culture was not acclimatised to the test substance or reference substance.

Test type:

static

Water media type:

freshwater

Total exposure duration:

16 h

Remarks on exposure duration:

±1 hour

Nominal and measured concentrations:

test concentrations ranged from 0.1 mg/L to 250 mg/L

Details on test conditions:

The composition of the reaction medium corresponded to the data given in the cited DIN guideline.
Incubation took place at 21±1°C in Erlenmeyer flasks over a period of 16±1 hours. For aeration, the solutions were kept in suspension on a rotary shaker with a circular movement, 5 cm in diameter, and at approx. 220 revolutions per minute. The composition of the reaction medium corresponded to the data given in the cited DIN guideline.
The sensitivity of the bacterial culture was checked with a reference batch. 2,4-dichlorophenol was used as reference batch in the concentrations of
30, 70 and 100 mg/l.

Reference substance (positive control):

yes

Remarks:

2,4-dichlorophenol

Duration:

16 h

Dose descriptor:

EC10

Effect conc.:

0.1 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth inhibition

Duration:

16 h

Dose descriptor:

EC50

Effect conc.:

2.3 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth inhibition

Results with reference substance (positive control):

2,4-dichlorphenol was used as reference batch in the concentrations of 30, 70 and 100 mg/L. The EC50 was between 30 and 100 mg/L.

Validity criteria fulfilled:

yes

Conclusions:

The test substance is toxic to microorganisms at an EC50 of 2.3 mg/L and an EC10 of 0.1 mg/L.

Executive summary:

The bacterial toxicity was investigated in a cell multiplication inhibition test with Pseudomonas putida in accordance with the guideline DIN 38 412, part 8. The test organism were exposed to concentration between 0.1 and 250 mg/L for 16 h. The effect concentration of the test substance was determined at 2.3 mg/L (EC50) and 0.1 mg/L (EC10).

Description of key information

The bacterial toxicity was investigated in a cell multiplication inhibition test with Pseudomonas putida in accordance with the guideline DIN 38 412, part 8. The test organism were exposed to concentration between 0.1 and 250 mg/L for 16 h. The effect concentration of the test substance was determined at 2.3 mg/L (EC50) and 0.1 mg/L (EC10).

Key value for chemical safety assessment

EC50 for microorganisms:

2.3 mg/L

EC10 or NOEC for microorganisms:

0.1 mg/L