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Diss Factsheets
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EC number: 201-741-4 | CAS number: 87-39-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Skin irritation / corrosion
Administrative data
- Endpoint:
- skin irritation: in vitro / ex vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 018
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 439 (In Vitro Skin Irritation: Reconstructed Human Epidermis Test Method)
- Deviations:
- no
- GLP compliance:
- no
- Remarks:
- The study has been performed in House according to current guidelines by trained technicians. The study is commonly performed at the lab and leaves up to the validty criteria.Thus data from the study can be considered reliable.
Test material
- Reference substance name:
- 5-hydroxyiminobarbituric acid
- EC Number:
- 201-741-4
- EC Name:
- 5-hydroxyiminobarbituric acid
- Cas Number:
- 87-39-8
- Molecular formula:
- C4H3N3O4
- IUPAC Name:
- 5-(hydroxyimino)-1,3-diazinane-2,4,6-trione
- Test material form:
- solid: particulate/powder
- Details on test material:
- Lot/batch No.: 201713807
Constituent 1
In vitro test system
- Test system:
- human skin model
- Source species:
- human
- Cell type:
- non-transformed keratinocytes
- Cell source:
- foreskin from a single donor
- Control samples:
- yes, concurrent positive control
- Amount/concentration applied:
- TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 16 mg
NEGATIVE CONTROL
- Amount(s) applied (volume or weight): 16µl PBS
POSITIVE CONTROL
- Amount(s) applied (volume or weight): 16 µl SDS
- Concentration (if solution): 50mg/mL - Duration of treatment / exposure:
- 42 min +/- 2 min
- Duration of post-treatment incubation (if applicable):
- 42h +/- 1h
- Number of replicates:
- 3
Results and discussion
In vitro
Results
- Irritation / corrosion parameter:
- % tissue viability
- Value:
- 1.3
- Vehicle controls validity:
- not applicable
- Negative controls validity:
- valid
- Positive controls validity:
- valid
- Remarks on result:
- positive indication of irritation
Applicant's summary and conclusion
- Interpretation of results:
- study cannot be used for classification
- Conclusions:
- Violuric was found to reduce the cell viabilty more than 50% in the present test system and must thus be considered as an irritant. However, the present test system cannot distinguish between category 1 and 2 classification.
- Executive summary:
Violuric acid has been tested for skin irritation according to OECD with the ' SkinEthicTMReconstructed Human Epidermis kit RHE/S/17'.
The irritation potential was assessed indirectly by assessing the uptake of MTT in living cells, which results in conversion to MTT in a blue dye, which can be measured spectophotometrically and by visual assessment of the tissue after 42 min exposure to the test substance followed by 42 h post treatment period. Viability of cells exposed to violuric acid was 1% compared to control cells and thus indicates irritaion potential of the test substance.
According to the test system violuric acid should be classified as irritant, but the test system cannot distinguish between category 1 and 2 classification.
In conclusion, violuric acid was found to be irritaing in the present test system.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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