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EC number: 200-372-6 | CAS number: 58-27-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: screening test, other
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 30 March 1995 - 19 June 1995
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 995
- Report date:
- 1995
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 302 C (Inherent Biodegradability: Modified MITI Test (II))
- Version / remarks:
- May 1981
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-F (Determination of the "Ready" Biodegradability - MITI Test)
- Version / remarks:
- 1992
- GLP compliance:
- yes
Test material
- Reference substance name:
- Menadione
- EC Number:
- 200-372-6
- EC Name:
- Menadione
- Cas Number:
- 58-27-5
- Molecular formula:
- C11H8O2
- IUPAC Name:
- 2-methyl-1,4-dihydronaphthalene-1,4-dione
- Test material form:
- solid: particulate/powder
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, non-adapted
- Details on inoculum:
- The inoculum was derived from ten different sites in Switzerland, including activated sludge from domestic and industrial sewage (seven sites) and surface water from three different rivers.
One liter of the various samples was collected and immediately aerated. After transportation, it was centrifuged and washed two times in mineral medium. Thereafter, the sludge was resuspended in mineral medium at a concentration of 3 to 5 g of dry sludge per liter. Finally, the suspension was stirred and aerated for 24 hrs prior to use. - Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- ca. 30 mg/L
- Based on:
- test mat.
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- An automated closed system oxygen consumption measuring apparatus (VOITH SAPROMAT®) was used. The machine has a control and display unit. Substances to be tested were inoculated in the testing vessels under aerobic conditions with microorganisms (activated sludge). The reaction processes consume the dissolved oxygen and generate CO2. Granular sodium lime, grain size 2-5 mm (Fluka, No. 72073), is used for CO2 adsorption. The sodium lime contains a color indicator to indicate a saturation with CO2. The resulting decrease in pressure is detected and converted into an electrical signal by means of an electrode type manometer. The consumed oxygen is replaced by electrolytically generated oxygen from a copper sulfate solution. Biodegradation is calculated by dividing the consumed oxygen by the theoretical oxygen demand of the substance. A control experiment using aniline as a reference substance was run in parallel. Furthermore, an inhibition test was included, where the test substance was incubated in the presence of the reference substance to determine a possible toxic effect of the test substance on activated sludge microorganisms.
Reference substance
- Reference substance:
- aniline
Results and discussion
- Preliminary study:
- none
- Test performance:
- The test substance was poorly water soluble and was therefore added directly into the test vessels.
Temperature (water bath): 25 °C
% Degradation
- Parameter:
- % degradation (O2 consumption)
- Value:
- 0
- Sampling time:
- 28 d
- Details on results:
- Theoretical oxygen demand (ThOD) of test substance: 2.23 mg O2/mg substance
BOD5 / COD results
- Results with reference substance:
- The biodegradation of the reference substance anilin was 70% after 7 days and 108% at the end of the test. The plateau was reached after 18 days.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- under test conditions no biodegradation observed
- Conclusions:
- The used inherent biodegradability test shows no evidence for biodegradation of menadione after 28 days of incubation with activated sludge. Furthermore, it is slightly toxic to microorganisms of activated sludge.
- Executive summary:
The theoretical oxygen demand for the reference substance is 2.41 mg O2/mg substance (corresponds to 241 mg O2
for 100 mg substance). The respective oxygen demand for the test substance is 2.23 mg O2/mg substance. This corresponds to 66.9 mg O2 for 30 mg test substance. These ThOD values were used to calculate the % degradation.
The biodegradation of the reference substance aniline was 70 % after 7 days and 108 % at the end of the test. The plateau was reached after 18 days.
The test substance showed no degradation after 28 days of testing. The negative values indicate, that respiration of the microorganisms in the presence of Menadione is considerably reduced compared to conditions without test compound.
When the reference substance was incubated in the presence of the test substance, degradation of the reference substance was slightly inhibited. The degradation of the reference substance under these conditions was 87 % at the end of the test.
As an inference, the used inherent biodegradability test shows no evidence for biodegradation of menadione after 28 days of incubation with activated sludge. Furthermore, it is slightly toxic to microorganisms of activated sludge.
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