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EC number: 611-575-8 | CAS number: 577953-88-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Long-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- long-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1999
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- other: OECD SIDS
- Title:
- Unnamed
- Year:
- 1 999
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: other guideline: OECD Guideline 202, part 2 "Daphnia sp., Reproduction Test" and the Draft Test Guideline 211 (April, 1997, OECD)
- Principles of method if other than guideline:
- Method: other: OECD Guideline 202, part 2 "Daphnia sp., Reproduction Test" and the Draft Test Guideline 211 (April, 1997, OECD)
- GLP compliance:
- yes
Test material
- Reference substance name:
- Dicyclohexylamine
- EC Number:
- 202-980-7
- EC Name:
- Dicyclohexylamine
- Cas Number:
- 101-83-7
- IUPAC Name:
- N-cyclohexylcyclohexanamine
- Test material form:
- liquid
Constituent 1
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
IUCLID4 Test substance: other TS: Dicyclohexylamine, CAS:100-74-3, Wako Pure Chemical Industries, Ltd., Lot. No.: WtK5069, purity = 99.9 %
Sampling and analysis
- Analytical monitoring:
- yes
Test solutions
- Vehicle:
- no
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- - Daphnids were cultured under the incubation conditions (see below) and fed with Chlorella vulgaris. Incubation at 20 +/- 1 °C during 16 h/8 h light/darkness regime, every 2-3 d exchange of incubation medium
Study design
- Test type:
- semi-static
- Water media type:
- not specified
Test conditions
- Hardness:
- - total hardness 55.2 mg/l CaCO3, containing (all in mg/l) COD <1, P <0.01, total Hg <0.0005, Cu <0.01, Cd <0.001, Zn <0.01, Pb <0.005, Al 0.03, Ni <0.02, total Cr <0.02, soluble Mn <0.005, Sn <0.03, soluble Fe <0.03, CN- <0.01, free Cl <0.01, Br- <0.5, F- 0.45, S2- <0.01, NH4+ <0.01, As <0.001, Se <0.001, Na 53, K 4.9, Ca 16, Mg 3.3, total chlorinated pesticides <0.00002, total organophosphorus pesticides <0.001, PCB <0.0005. Total hardness was determined in the incubation media several times during the assays and was found to be 60-80 mg/l
- Test temperature:
- Incubation at 20 +/- 1 °C
- pH:
- Dilution water, pH 8.1, pH was monitored at start and at the end of the incubations. pH was 7.8-8.2 during incubation
- Dissolved oxygen:
- - Oxygen concentration was 7.4-8.6 mg/l during incubation
- Details on test conditions:
- Incubation at 20 +/- 1 °C during 16 h/8 h light/darkness regime, every 2-3 d exchange of incubation medium
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.016 mg/L
- Nominal / measured:
- meas. (TWA)
- Basis for effect:
- reproduction
- Duration:
- 21 d
- Dose descriptor:
- LOEC
- Effect conc.:
- 0.049 mg/L
- Nominal / measured:
- meas. (TWA)
- Basis for effect:
- reproduction
- Duration:
- 21 d
- Dose descriptor:
- EC50
- Effect conc.:
- 0.14 mg/L
- Nominal / measured:
- meas. (TWA)
- Basis for effect:
- reproduction
- Duration:
- 21 d
- Dose descriptor:
- LC50
- Effect conc.:
- 1.3 mg/L
- Nominal / measured:
- meas. (TWA)
- Basis for effect:
- mortality
- Remarks on result:
- other: (95 % confidence limits: 0.54-10 mg/l, Probit)
- Reported statistics and error estimates:
- Statistical method: For LC50 Probit and EcoTox-Statics Release 1.1, for EC50 Logistic and EcoTox-Statics Release 1.1, and for NOEC and LOEC Non-parametric Dunnett and EcoTox-Statics Release 1.1.
Any other information on results incl. tables
21 d-LC50 for mortality of parent Daphnias was 1.3 mg/l (95
% confidence limits: 0.54-10 mg/l, Probit). In detail, the
cumulative mortality of parental Daphnias (%) was:
7d 14d 21d
Control 0 0 0
0.016 0 0 0
0.049 0 0 10
0.23 0 20 20
0.80 0 10 30
2.7 10 20 70
21 d-EC50 for inhibition of reproduction was 0.14 mg/l
(Logistic). In detail, the mean cumulative numbers of
juveniles produced per adult were:
7d 14d 21d
Control 2.2 46.8 114.9
0.016 4.4 36.6 135.2
0.049 3.6 37.3 91.6
0.23 2.4 14.8 44.4
0.80 1.1 2.3 13.3
2.7 0.0 0.0 0.0
Reproduction was slightly increased at 0.016 mg/l, but
completely inhibited at 2.7 mg/l:
Conc. Control 0.016 0.049 0.23 0.80 2.7
Mean 114.9 135.2 91.6 44.4 13.3 0.0
S.D. 35.4 43.2 37.8 11.3 13.2 0.0
Inhibition (%) -18 20 61 88 100
The time (d) to first brood production was not influenced by
cyclohexylamine up to 0.049 mg/l:
Vessel Control 0.016 0.049 0.23 0.80 2.7
1 7 7 7 7 8 -
2 7 7 7 9 8 -
3 7 7 7 7 8 -
4 7 7 7 8 8 -
5 7 7 7 7 8 -
6 7 7 7 8 8 -
7 8 7 7 8 8 >21
8 8 7 8 8 8 >21
9 7 8 7 7 7 >21
10 7 7 7 7 8 -
Mean 7.2 7.1 7.1 7.6 7.9 >21
Effect Data(Reproduction):
LC50 (21d) = 1.3 (0.54-10) mg/ml (parental mortality) (mc)
EC50 (21d) = 0.14 mg/L (Reproduction) (mc)
NOEC (21d) = 0.016 mg/L (Reproduction) (mc)
LOEC (21d) = 0.049 mg/L (Reproduction) (mc)
mc: based on time-weighted mean of measured concentrations
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The following results were reported for daphnia magna:
Effect Data (Reproduction): LC50 (21d) = 1.3 mg/ml (parental mortality); EC50 (21d) = 0.14 mg/L (Reproduction); NOEC (21d) = 0.016 mg/L (Reproduction) and LOEC (21d) = 0.049 mg/L (Reproduction)
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