Sorbitan, tridocosanoate

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Study period: 15 - 17 Nov 2017

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes

Analytical monitoring:

yes

Details on sampling:

- Concentrations: 100 mg/L, sampled at test start end end of exposure period.

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test sample (0.100 g) and dilution water (1000 mL) were mixed to produce the nominal concentration of 100 mg/L, and they were stirred with a magnetic stirrer for 48 hours at about 20°C to prepare the test solution.
The test solution in the exposure level was white suspended at the start of exposure. At the end of exposure, the test solution in the exposure level was slightly white suspended and precipitation and insoluble matter were observed.

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Water flea
- Source: The University of Sheffield, UK
- Age of parental stock (mean and range, SD): 14 d
- Feeding during test: no


ACCLIMATION
- Acclimation period: 14 d
- Acclimation conditions (same as test or not): same
- Type and amount of food: Chlorella vulgaris of 0.1- 0.2 mgC (Organic carbon content)/day per Daphnia once a day.

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Hardness:

32 mg/L

Test temperature:

19.9 - 20.1 °C

pH:

7.8 - 7.9

Dissolved oxygen:

8.8 - 9.0 mg/L

Nominal and measured concentrations:

Nominal test substance concentration: 100 mg/L
Measured test substance concentration: < LOD of 0.521 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: glass beaker (100 mL)
- Type (delete if not applicable): covered with transparent plastic lid
- Fill volume: 100 mL
- Aeration: no aeration
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Dechlorinated Tap water
- Total organic carbon: < 0.5 mg/L
- Conductivity: 13 mS/m

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16:8 h day-night regime

EFFECT PARAMETERS MEASURED (with observation intervals if applicable)
- Mobility after 24 and 48 h

Reference substance (positive control):

yes

Remarks:

Potassium dichromate

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

- Behavioural abnormalities: none observed
- Mortality of control: none
Since the concentration of the test item in the test solution during exposure was less than the determination limit value ( <0.536 mg/L), neither dissolution concentration nor the stability in the exposure period was clear. However, no effects on test organisms were observed, indicating that the test item exert no acute effect on test organisms at around the solubility in dilution water.

Results with reference substance (positive control):

Latest test date: 18 - 20 October 2017
48-hour EC50: 0.12 mg/L (mean± 2 S.D.: 0.10-0.35 mg/L,n= 102)

Table 1: Survival of D. magna.

Nominal concentration [mg/L]	Test Vessel	Number of immobilised daphnids/Total daphnids after 48 h	Immobility [%]
Control	A	0/5	0
	B	0/5
	C	0/5
	D	0/5
100	A	0/5	0
	B	0/5
	C	0/5
	D	0/5

Table 2: Validity criteria for OECD 202.

Criterion from the guideline	Outcome	Validity criterion fulfilled
In the control, including the control containing the solubilising agent, not more than 10% of the daphnids should have been immobilized.	0% immobilised	yes
The dissolved oxygen concentration at the end of the test should be ≥ 3 mg/L in control and test vessels.	8.8 - 9.0 mg/L	yes

 

Validity criteria fulfilled:

yes

Remarks:

For further details please refer to “Any other information on results incl. tables”.

Description of key information

EC50 (48 h) >100 mg/L (nominal)   

Additional information

The acute toxicity of the test substance to aquatic invertebrates was tested in a study following OECD guideline 202 (GLP). Daphnia magna were exposed to a nominal test substance concentration of 100 mg/L in a static limit test. The nominal concentration was analytically verified by HPLC. The test substance concentration was below the determination limit of the method of 0.521 mg/L reflecting the very low water solubility of the substance of 0.525 mg/L at 20 °C, pH 5.9.

During the exposure period of 48 hours mortality or behavioural abnormalities were not observed. The reported EC50 (48 h) is >100 mg/L (nominal).  

Based on the results acute toxic effects on aquatic invertebrates are not expected within the range of water solubility.