Reaction mass of Cobaltate(2-), [2-[[[4-hydroxy-3-[[2-oxo-1-[(phenylamino)carbonyl]propyl]azo]phenyl]sulfonyl]amino]benzoato(3-)][2-[[2-hydroxy-5-[(phenylamino)sulfonyl]phenyl]azo]-3-oxo-N-phenylbutanamidato(2-)]-, disodium and Cobaltate(3-), bis[2-[[[4-hydroxy-3-[[2-oxo-1-[(phenylamino)carbonyl]propyl]azo]phenyl]sulfonyl]amino]benzoato(3-)]-, trisodium and sodium bis[2-[[2-hydroxy-5-[(phenylamino)sulphonyl]phenyl]azo]-3-oxo-N-phenylbutyramidato(2-)]cobaltate(1-)

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Remarks:

source of read across

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

no

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: daphnia.
- Age of organisms: <72 hours of age.

BREEDING
- Breeding conditions: animals are bred under temperature - and light- controlled conditions in water containing sufficient microorganisms. Temperature 21 ± 2 °C; light from 06:00 am to 6:00 pm (12 h light/dark cycle).
- Water: breeding water is prepared with 0.2 g of NaHCO3, 0.224 g of CaCl2, 0.026 g of K2SO4 filled up with distilled water to 1000 ml. Alternate: “Evian” water.
- Feeding frequency: 2 or 3 times a week.

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test temperature:

Vessels: 21 °C
Control: 21 °C

pH:

Vessels: 7.6 - 7.8
Control: 7.6 - 7.7

Dissolved oxygen:

Vessels:saturated - 96 %
Control: saturated - 99 %

Nominal and measured concentrations:

Nominal concentrations : 0.1,1.0, 10 and 100 mg/l

Details on test conditions:

TEST SYSTEM
- No. of organisms per vessel: 5 daphnids.
- No. of vessels per concentration: 2 replicates.
- No. of vessels per control: 2 replicates.

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: test tubes are filled with 9 ml of “Evian” water each.

EFFECT PARAMETERS MEASURED
After 24h and 48h respectively mobile (living) daphnids are counted.

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

No immobilization was noted in all the test concentrations at the end of 48 h exposure period.

OVERALL IF RESULTS

Nominal concentrations (mg/l)	No. of animals	No. of immobilized Dapnhia (24 h)	No. of immobilized Dapnhia (48 h)	% immobility (24 h)	% immobility (48 h)
Vehicle	5 + 5	0	0	0	0
Blank	5 + 5	0	0	0	0
0.1	5 + 5	0	0	0	0
1.0	5 + 5	0	0	0	0
10	5 + 5	0	0	0	0
100	5 + 5	0	0	0	0

TEST CONDITIONS

Nominal conc. (mg/l)	Start	End
	100	Vehicle	Blank	0.1	1	10	100
Oxygen (%)	Saturated	99	99	97	97	97	96
pH	7.6	7.7	7.7	7.8	7.8	7.8	7.8
Temperature (°C)	21	21	21	21	21	21	21

Conclusions:

EC50 (48h) > 100 mg/l (nominal)

Executive summary:

Toxicity of test substance was estimated according to OECD test guideline 202. Five daphnids in duplicate sets per test concentration were exposed to 0.1, 1.0, 10 and 100 mg/l concentration . No immobilization was noted in all the test concentrations at the end of 48 h exposure period; therefore, the 48 h EC50 was found to be greater than 100 mg/l.

Conclusion

EC50 (48h) > 100 mg/l (nominal)

Description of key information

Not harmful/toxic for invertebrates (EC50 (48h) > 100 mg/l (nominal))

Additional information

There is no information about the short-term toxicity to invertebrates of Acid Yellow 235, thus the available data on structural analogous Similar Substance 01 has been taken into account. The read across approach can be considered as reliable and adequate for the purpose; details and explanations are detailed in the report attached to the IUCLID section 13.2.

Toxicity of Similar Substance 01 was estimated according to OECD test guideline 202. Five daphnids in duplicate sets per test concentration were exposed to 0.1, 1.0, 10 and 100 mg/l concentration . No immobilization was noted in all the test concentrations at the end of 48 h exposure period; therefore, the 48 h EC50 was found to be greater than 100 mg/l.