Iron tris(phosphinate)

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Remarks:

for read across substance

Justification for type of information:

Data for the target chemical is summarized based on the structurally similar read across chemicals

Reason / purpose for cross-reference:

read-across source

Reason / purpose for cross-reference:

read-across source

Qualifier:

according to guideline

Guideline:

other: as mentioned below

Principles of method if other than guideline:

WoE report is based on two short term toxicity to aquatic invertebrate for the test chemical :
1.Acute immobilization of Daphnia was performed for test material
2.Acute toxicity testing of test matrial on Daphnia magna using the 48 h Acute Immobilisation Test

GLP compliance:

not specified

Specific details on test material used for the study:

- Name of test material: Ferric hypophosphite
- IUPAC name: iron(3+) ion triphosphinate
- Molecular formula: FeO6P3
- Molecular weight: 250.8084 g/mole
- Smiles : [Fe+3].[O-]P=O.[O-]P=O.[O-]P=O
- Inchl: 1S/Fe.3H3O2P/c;3*1-3-2/h;3*3H2,(H,1,2)/q+3;;;/p-3
- Substance type: Inorganic
- Physical state: Solid powder (white to grey)

Analytical monitoring:

not specified

Vehicle:

not specified

Test organisms (species):

Daphnia magna

Details on test organisms:

1 . <72 h old neonates from the acute
immobilization tests.

20 actively swimming neonates from each test concentration were
collected and transferred into clean lake water
- Type and amount of food: Pseudokirchneriella subcapitata
- Frequency: once per day

2.Age: <24 h old, 4 replicates of 5 neonates
20 animals were tested

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Nominal and measured concentrations:

1.10 mg/l , 100 mg/l , 1000 mg/l
2.0.01, 0.1, 1, 10 and 100 mg /L

Details on test conditions:

1.Every individual was exposed separately in a 40 ml vessel

Reference substance (positive control):

yes

Remarks:

K2Cr2O7 for Read across 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: Read across 1

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: Read across 2

Validity criteria fulfilled:

not specified

Conclusions:

The test chemical Ferric hypophosphite is not likely to be toxic to Daphnia magna atleast in the dose range of 100-1000 mg/l

Executive summary:

Data available for the structurally and functionally similar read across chemicals has been reviewed to determine the short term toxicity of Daphnia magna of the test chemical Ferric hypophosphite (7783 -84 -3).The studies are as mentioned below:

1. The acute immobilization of Daphnia magna was performed using test material for 48h.The acute immobilization tests showed that in general the test material were not acutely toxicto D. magna: even at 100 ppmDue to the low toxicity of magnetite, we also evaluated the toxic

effects at 1000 ppm although according to the test guidelines (OECD). However, even at 1000 ppm the 50% inhibitory effect was not observed. Hence , The effect concentration was observed to be > 1000 mg/l.

2.The immobilization test of Daphnia magna was performed for test material as per OECD 202 . Groups of 20 water fleas, Daphnia magna, (< 24 h old, 4 replicates of 5 neonates) were exposed to uncontaminated water, 0.01, 0.1, 1, 10 and 100 mg as/L under static conditions in a range finding test.The duration of the test was 48 h.The effect concentration of test material on Daphnia magna was observed to be >100 mg/l.

Thus, based on the above summarised studies, Ferric hypophosphite and it’s structurally and functionally similar read across substance, it can be concluded that effect concetration value is greater than 100 mg/L. Thus, comparing this value with the criteria of CLP regulation,Ferric hypophosphite cannot be classifiedfor short term toxicity for aquatic invertebrates .Hence,based on the data available for the structurally and functionally similar

read across, test chemical Ferric hypophosphite is not likely to be toxic atleast in the dose range of 100 mg/L .

 

Description of key information

Short-term toxicity to aquatic invertebrates:

Data available for the structurally and functionally similar read across chemicals has been reviewed to determine the short term toxicity of Daphnia magna of the test chemical Ferric hypophosphite (7783 -84 -3).The studies are as mentioned below:

1. The acute immobilization of Daphnia magna was performed using test material for 48h.The acute immobilization tests showed that in general the test material were not acutely toxicto D. magna: even at 100 ppmDue to the low toxicity of magnetite, we also evaluated the toxic

effects at 1000 ppm although according to the test guidelines (OECD). However, even at 1000 ppm the 50% inhibitory effect was not observed. Hence , The effect concentration was observed to be > 1000 mg/l.

2.The immobilization test of Daphnia magna was performed for test material as per OECD 202 . Groups of 20 water fleas, Daphnia magna, (< 24 h old, 4 replicates of 5 neonates) were exposed to uncontaminated water, 0.01, 0.1, 1, 10 and 100 mg as/L under static conditions in a range finding test.The duration of the test was 48 h.The effect concentration of test material on Daphnia magna was observed to be >100 mg/l.

Thus, based on the above summarised studies, Ferric hypophosphite and it’s structurally and functionally similar read across substance, it can be concluded that effect concetration value is greater than 100 mg/L. Thus, comparing this value with the criteria of CLP regulation,Ferric hypophosphite cannot be classifiedfor short term toxicity for aquatic invertebrates .Hence,based on the data available for the structurally and functionally similar

read across, test chemical Ferric hypophosphite is not likely to be toxic atleast in the dose range of 100 mg/L .

 

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

1 000 mg/L

Additional information

Short-term toxicity to aquatic invertebrates:

Data available for the structurally and functionally similar read across chemicals has been reviewed to determine the short term toxicity of Daphnia magna of the test chemical Ferric hypophosphite (7783 -84 -3).The studies are as mentioned below:

1. The acute immobilization of Daphnia magna was performed using test material for 48h.The acute immobilization tests showed that in general the test material were not acutely toxicto D. magna: even at 100 ppmDue to the low toxicity of magnetite, we also evaluated the toxic

effects at 1000 ppm although according to the test guidelines (OECD). However, even at 1000 ppm the 50% inhibitory effect was not observed. Hence , The effect concentration was observed to be > 1000 mg/l.

2.The immobilization test of Daphnia magna was performed for test material as per OECD 202 . Groups of 20 water fleas, Daphnia magna, (< 24 h old, 4 replicates of 5 neonates) were exposed to uncontaminated water, 0.01, 0.1, 1, 10 and 100 mg as/L under static conditions in a range finding test.The duration of the test was 48 h.The effect concentration of test material on Daphnia magna was observed to be >100 mg/l.

Thus, based on the above summarised studies, Ferric hypophosphite and it’s structurally and functionally similar read across substance, it can be concluded that effect concetration value is greater than 100 mg/L. Thus, comparing this value with the criteria of CLP regulation,Ferric hypophosphite cannot be classifiedfor short term toxicity for aquatic invertebrates .Hence,based on the data available for the structurally and functionally similar

read across, test chemical Ferric hypophosphite is not likely to be toxic atleast in the dose range of 100 mg/L .