Nitrilotrimethylenetris(phosphonic acid)

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Study period:

No data

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference

Reference Type:

other company data

Title:

Unnamed

Year:

1967

Report date:

1967

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 401 (Acute Oral Toxicity)

Version / remarks:

Conducted prior to the adoption of OECD test guidelines.

Deviations:

yes

Remarks:

Limited detail on test substance, and methodology.

GLP compliance:

no

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Nitrilotrimethylenetris(phosphonic acid)

EC Number:

229-146-5

EC Name:

Nitrilotrimethylenetris(phosphonic acid)

Cas Number:

6419-19-8

Molecular formula:

C3H12NO9P3

IUPAC Name:

nitrilotrimethylenetris(phosphonic acid)

Test animals

Species:

rat

Strain:

Sprague-Dawley

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: No data
- Age at study initiation: No data
- Weight at study initiation: Males: 230 - 250 g. Females: 210 - 225 g.
- Fasting period before study: No data
- Housing: No data
- Diet (e.g. ad libitum): No data
- Water (e.g. ad libitum): No data
- Acclimation period: No data


ENVIRONMENTAL CONDITIONS
- Temperature (°C): No data
- Humidity (%): No data
- Air changes (per hr): No data
- Photoperiod (hrs dark / hrs light): No data


IN-LIFE DATES: No data

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

water

Details on oral exposure:

MAXIMUM DOSE VOLUME APPLIED: No data.

Doses:

2000, 2510, 3160 and 3980 mg/kg bw (as a 25 % aqueous solution).

No. of animals per sex per dose:

Two or three depending on dose (five in total/dose).

Control animals:

no

Details on study design:

- Duration of observation period following administration: No data, but at least four days.
- Frequency of observations and weighing: Not stated
- Necropsy of survivors performed: No
- Other examinations performed: clinical signs, and gross pathology.

Statistics:

LD50 calculated by a modification of the method of E. J. de Beer (no further detail, calculation not presented).

Results and discussion

Effect levels

Key result

Sex:

male/female

Dose descriptor:

LD50

Effect level:

2 910 mg/kg bw

Based on:

act. ingr.

Remarks:

active acid

95% CL:

>= 2 530 - <= 3 345

Mortality:

No deaths at the lowest dose of 2000 mg/kg bw. There were one, two and five deaths in the 2510, 3160 and 3980 mg/kg bw groups, respectively. Survival time was several hours to four days, with most death occurring "overnight" (it is not clear if this means the night after the dosing).

Clinical signs:

other: Weakness in one to two hours with diarrhoea, salivation and tremors.

Gross pathology:

Inflammation of the gastrointestinal mucosa as well as liver and renal hyperaemia.

Other findings:

None

Applicant's summary and conclusion

Interpretation of results:

GHS criteria not met

Conclusions:

In the acute oral toxicity study, conducted according to a protocol similar to the now-deleted OECD Test Guideline 401 and prior to GLP, an LD50 of 2910 mg active acid/kg bw (the doses were corrected for purity) was determined for ATMP-H (aqueous solution containing 25% active acid) in the rat.