Nitrilotrimethylenetris(phosphonic acid)

Acute Toxicity: dermal

Administrative data

Endpoint:

acute toxicity: dermal

Type of information:

experimental study

Adequacy of study:

key study

Study period:

No data

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference

Reference Type:

other company data

Title:

Unnamed

Year:

1967

Report date:

1967

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 402 (Acute Dermal Toxicity)

Version / remarks:

Conducted prior to adoption of OECD test guidelines.

Deviations:

yes

Remarks:

Limited details on methods and animal conditions.

Principles of method if other than guideline:

Method: other: Not stated. Insufficient detail to fully assess comparability with OECD guideline.

GLP compliance:

no

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Nitrilotrimethylenetris(phosphonic acid)

EC Number:

229-146-5

EC Name:

Nitrilotrimethylenetris(phosphonic acid)

Cas Number:

6419-19-8

Molecular formula:

C3H12NO9P3

IUPAC Name:

nitrilotrimethylenetris(phosphonic acid)

Test animals

Species:

rabbit

Strain:

New Zealand White

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: No data
- Age at study initiation: No data
- Weight at study initiation: Males: 2.3-2.5 kg. Females: 2.0 and 2.1 kg.
- Fasting period before study: No data
- Housing: Individually (cage type to stated)
- Diet (e.g. ad libitum): No data
- Water (e.g. ad libitum): No data
- Acclimation period: No data


ENVIRONMENTAL CONDITIONS
- Temperature (°C): No data
- Humidity (%): No data
- Air changes (per hr): No data
- Photoperiod (hrs dark / hrs light): No data


IN-LIFE DATES: No data

Administration / exposure

Type of coverage:

occlusive

Vehicle:

water

Details on dermal exposure:

TEST SITE
- Area of exposure: No data
- % coverage: No data
- Type of wrap if used: "plastic strips"


REMOVAL OF TEST SUBSTANCE
- Washing (if done): No data


TEST MATERIAL
- Amount(s) applied (volume or weight with unit): No data
- Concentration (if solution): 25% aqueous solution
- Constant volume or concentration used: yes


VEHICLE
- Amount(s) applied (volume or weight with unit): No data
- Concentration (if solution): 25% solution

Duration of exposure:

24 hours

Doses:

1000, 1580, 2510, 3980 and 6310 mg/kg bw

No. of animals per sex per dose:

One

Control animals:

no

Details on study design:

- Duration of observation period following administration: Not clear, but at least five days.
- Frequency of observations and weighing: Body weights appear to have been measured prior to dosing and five days after dosing.
- Necropsy of survivors performed: no
- Other examinations performed: clinical signs and gross pathology of animals that die.

Statistics:

None

Results and discussion

Effect levels

Key result

Sex:

male/female

Dose descriptor:

LD50

Effect level:

> 6 310 mg/kg bw

Based on:

act. ingr.

Remarks:

active acid

Mortality:

No deaths occurred at any dose.

Clinical signs:

other: Activity and appetite were temporarily reduced after doses of 3980 and 6310 mg/kg bw.

Gross pathology:

No deaths so not conducted.

Other findings:

None

Applicant's summary and conclusion

Interpretation of results:

GHS criteria not met

Conclusions:

In the key acute dermal toxicity study conducted prior to the adoption of OECD test guidelines and GLP, but according to a protocol similar to OECD Test Guideline 402, the LD50 for ATMP-H (aqueous solution containing 25% w/w active acid) was concluded to be >6310 mg active acid/kg bw.