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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
other: read accross of critical study record from specific substance (CdCl2) driving classification
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Acceptable, well documented publication that meets basic scientifc principles, similar to standard

Data source

Reference
Reference Type:
publication
Title:
Differences in acute toxicity test results of three reference toxicants on Daphnia at two temperatures
Author:
Lewis PA and Horning II WB
Year:
1991
Bibliographic source:
Environmental Toxicology and Chemistry, 10, 1351-1357.

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
other: EPA 600/4-78 012
Deviations:
not specified
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
CdCl2

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
no
Details on test solutions:
Stock solution of 72mg/L Cd as CdCl2 was prepared and kept in the refrigerator

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Age at study initiation (mean and range, SD): neonates
- Feeding during test
- Food type: fish food solution
- Amount: 0.3ml/vessel
- Frequency: once at beginning of test

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h

Test conditions

Hardness:
160-180 mg CaCO3/L
Test temperature:
20°C
pH:
8.5
Nominal and measured concentrations:
control and 3, 9, 27, 81, 243 and 729 µg Cd/L; 10 replicates
nominal equivalenty to measured concentrations
Reference substance (positive control):
no

Results and discussion

Effect concentrations
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
38 µg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
dissolved
Basis for effect:
mortality
Reported statistics and error estimates:
LC50 and confidence limits were calculated by moving average angle method

Any other information on results incl. tables

none

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
Tests done according to a standard protocol thus of good quality and considered useful for setting the reference value for acute aquatic ecotoxicity.
Executive summary:

Tests done according to a standard protocol, of good quality and considered useful for setting the reference value for acute aquatic ecotoxicity. Study used for that purpose in the EU risk assessment process on cadmium (ECB 2008). Culture medium, test water conditions well described and relevant the purpose of classification.