Sodium 3-(allyloxy)-2-hydroxypropanesulphonate

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Study period:

15.-24.5.2012

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

no

Vehicle:

no

Test organisms (species):

activated sludge of a predominantly domestic sewage

Details on inoculum:

- Laboratory culture: sludge taken from activation basin of the STP D-67435 NW-Lachen-Speyerdorf
- Method of cultivation:
- Preparation of inoculum for exposure:
- Pretreatment: filtration, washing with tap water followed by resuspension and aeration until usage in the test
- Initial biomass concentration: sludge: 3.18 g/L dry weight, test: 1.59 g/L dry weight

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

3 h

Hardness:

1.08 mmol/L

Test temperature:

21.3-22.5 °C

pH:

8.18

Nominal and measured concentrations:

nominal: 3820, 382, 38.2, 3.82 mg/L, respectively 1452, 145, 14.5, 1.45 mg a.i./L

Reference substance (positive control):

yes

Remarks:

3,5-dichlorophenol

Duration:

3 h

Dose descriptor:

EC50

Effect conc.:

> 1 452 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

growth inhibition

Duration:

3 h

Dose descriptor:

NOEC

Effect conc.:

1 452 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

growth inhibition

Duration:

3 h

Dose descriptor:

other: EC20

Effect conc.:

> 1 452 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

growth inhibition

Results with reference substance (positive control):

EC50 (3h) = 8.2 mg/L (95 c.i. > 12 mg/L)
The value lies within the recommendated range for 3,5-dichlorophenol.

Reported statistics and error estimates:

t-test

Table 1:content of test vessels

Number	Content	Nominal concentration (mg/L)	Nutrient Solution (mL)	H2O (mL)	Stock solution added (mL)	Concentration of stock solution (mg/L)	Inoculum (mL)
1	control	0	16	234	0	--	250
2	control	0	16	234	0	--	250
3	positive control	5	16	229	5	500	250
4	positive control	10	16	224	10	500	250
5	positive control	20	16	214	20	500	250
6	positive control	40	16	194	40	500	250
7	control	0	16	234	0	--	250
8	control	0	16	234	0	--	250
9	test item	3820	16	134	100	19100	250
10	test item	3820	16	134	100	19100	250
11	test item	3820	16	134	100	19100	250
12	test item	3820	16	134	100	19100	250
13	test item	3820	16	134	100	19100	250
14	test item	382	16	224	10	19100	250
15	test item	38.2	16	233	1	19100	250
16	test item	3.82	16	234	0.1	19100	250
17	control	0	16	234	0	--	250
18	control	0	16	234	0	--	250
19-22	*	*	*	*	*	*	*
23	control	0	16	234	0	--	250
24	control	0	16	234	0	--	250

* other test items were measured in vessels 19-22

 

Table 2:O₂-consumption, inhibition pre-test

Vessel	Content	Nominal concentration (mg/L)	O2consumption (mg/(L x min)	O2consumption (mg/(L x h)	Inhibition (%)	pH
1	control	0	0.5922	35.529	0	8.0
2	control	0	0.5886	35.315	0	8.0
3	positive control	5	0.4213	25.276	32.5	8.1
4	positive control	10	0.2649	15.895	57.5	8.1
5	positive control	20	0.1285	7.711	79.4	8.2
6	positive control	40	0.0815	4.892	86.9	8.2
7	control	0	0.6029	36.175	0	8.0
8	control	0	0.6167	37.001	0	8.0
9	test item	3820	0.5953	35.721	4.6	8.3
10	test item	3820	0.5864	35.182	6.0	8.3
11	test item	3820	0.6011	36.063	3.7	8.3
12	test item	3820	0.6098	36.588	2.3	8.2
13	test item	3820	0.6121	36.723	1.9	8.2
14	test item	382	0.6357	38.143	-1.9	8.0
15	test item	38.2	0.6196	37.175	0.7	8.0
16	test item	3.82	0.6713	40.279	-7.6	7.8
17	control	0	0.6513	39.078	0	7.9
18	control	0	0.6566	39.397	0	7.8
19-22	*	*	*	*	*	*
23	control	0	0.6298	37.787	0	8.0
24	control	0	0.6541	39.244	0	7.9

* other test items were measured in vessels 19-22

Validity criteria fulfilled:

yes

Conclusions:

The NOEC under test cocentration is 1452 mg a.i./L.

Executive summary:

In the conducted test, the test item was tested using five concentrations (ranging from 3820 to 3.82 mg/L test item resp. 1452 to 1.45 mg act. ingr./L HAPS). Duration of the test was three hours. Activated sludge was used as inoculum. It was taken from a domestic sewage treatment plant and washed before usage. The dry matter was determined as 3.18 g suspended solids/L, giving a concentration of 1.59 g suspended solids/L in the test.

3,5 -Dichlorophenol was used as positive control. Five concentrations were tested; a 3h-EC50 of 8.2 mg/L (95% c.i.: 3h-EC50 < 12 mg/L) was determined, which lies within the demanded range of 2 -25 mg/L.

As inhibition was below 10 % in the highest treatment (3820 mg/L, using five replicates) and no statistically significant difference to the control was observed, this concentration was stated as the NOEC and no second experiment was performed.

Description of key information

No significant inhibtion was observed at highest test concentration. Thus, NOEC is stated on the concentration of the active ingredient HAPS.

Key value for chemical safety assessment

EC10 or NOEC for microorganisms:

1 452 mg/L