Tetrachlorophthalic anhydride

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Guideline study, non GLP

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1989

Report date:

1989

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: German guideline (DIN 38 412/9).

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Tetrachlorophthalic anhydride

EC Number:

204-171-4

EC Name:

Tetrachlorophthalic anhydride

Cas Number:

117-08-8

Molecular formula:

C8Cl4O3

IUPAC Name:

tetrachloro-1,3-dihydro-2-benzofuran-1,3-dione

Details on test material:

- Name of test material (as cited in study report): Tetrachlorophthalic anhydride
- Analytical purity: purity not specified.

Sampling and analysis

Analytical monitoring:

no

Test organisms

Test organisms (species):

Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)

Details on test organisms:

TEST ORGANISM
- Source (laboratory, culture collection): Algae collection Goettingen, SAG 86.81

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

72 h

Test conditions

Test temperature:

20 °C

pH:

6.3-9.35

Nominal and measured concentrations:

Nominal test concentrations (mg/l):
0.39; 0.78; 1.56; 3.12; 6.25; 12.5; 25; 50; 100

Details on test conditions:

TEST SYSTEM
- Test vessel: glass tubes
- Fill volume: 10 ml test volume
- Initial cells density: 10 000 cells/ml
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: Chlorophyll-a-fluorescence (as measure for cell number) after 0, 24, 72 hours

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

98.9 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 2

Duration:

72 h

Dose descriptor:

EC20

Effect conc.:

47.5 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Any other information on results incl. tables

Concentration          % of Control  
  (mg/l)    

  0.39                  152.3
  0.78                  128.85
  1.56                  142.39
  3.125                 136.33
  6.25                  116.53
 12.5                    97.55
 25.0                    89.84
 56.0                    78.9
166.0                    49.32