2,9-bis[4-(phenylazo)phenyl]anthra[2,1,9-def:6,5,10-d'e'f']diisoquinoline-1,3,8,10(2H,9H)-tetrone

Acute Toxicity: other routes

Administrative data

Endpoint:

acute toxicity: other routes

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1978

Report date:

1978

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

internal test method

GLP compliance:

no

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

2,9-bis[4-(phenylazo)phenyl]anthra[2,1,9-def:6,5,10-d'e'f']diisoquinoline-1,3,8,10(2H,9H)-tetrone

EC Number:

221-264-5

EC Name:

2,9-bis[4-(phenylazo)phenyl]anthra[2,1,9-def:6,5,10-d'e'f']diisoquinoline-1,3,8,10(2H,9H)-tetrone

Cas Number:

3049-71-6

Molecular formula:

C48H26N6O4

IUPAC Name:

2,9-bis[4-(phenyldiazenyl)phenyl]isoquino[4',5',6':6,5,10]anthra[2,1,9-def]isoquinoline-1,3,8,10(2H,9H)-tetrone

Details on test material:

- Chemical name (as cited in study report): Perylen-3,4,9,10-tetracarbonsäure-bis-(4-phenylazo)-phenyl-imid
Test materials used in this dossier are all considered to fall under the definition of nano-materials according to the European Commission Recommendation 2011/696/EU as the synthesis and manufacturing of this pigment always yields particulate material with a fine particle size distribution.

Test animals

Species:

mouse

Strain:

other: NMRI-Wiga

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Weight at study initiation: average weight males: 26 g, females: 24 g
- Diet (e.g. ad libitum): HERILAN MRH-Haltung Alleinfutter für die Haltung von Mäusen, Ratten und Hamstern, Heinrich Eggersmann KG, Rinteln
- Weight at study initiation:
- Fasting period before study:
-

Administration / exposure

Route of administration:

intraperitoneal

Vehicle:

CMC (carboxymethyl cellulose)

Remarks:

0.5 % aequous solution

Details on exposure:

VEHICLE
- Concentration in vehicle: 20% supsension in 0.5% aqueous solution with carboxymethyl cellulose (w/v)

Doses:

2000 mg/kg bw

No. of animals per sex per dose:

5

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight

Results and discussion

Effect levels

Sex:

not specified

Dose descriptor:

LD50

Effect level:

> 2 000 mg/kg bw

Based on:

test mat.

Remarks on result:

other: no mortality observed

Mortality:

No mortalities occured.

Clinical signs:

Dyspnea, apathy, atony, spastic gait, ruffled fur. All symptoms reversed back to normal within 5 days.

Body weight:

ne effects reported

Gross pathology:

Intra-abdominal substance depositions

Applicant's summary and conclusion

Conclusions:

Under the conditions chosen, the test substance did not induce mortality in rats exposed intraperitoneally. Thus, the LD50 was concluded to be greater than 2000 mg/kg bw.