Registration Dossier

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Guideline study, non-GLP, limitations in reporting but otherwise adequate for assessment.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1990
Report Date:
1990

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
GLP compliance:
not specified

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): MBDA

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no
Details on test solutions:
A stock solution of 500 mg/L was prepared after that it was diluted to the appropriate test concentrations

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
Source: Institute national de recherche chimique appliquee, France. The stem culture has been in the BASF ecology lab since 1978

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h

Test conditions

Hardness:
total: 2.7 ± 0.5 mmol/L
Test temperature:
20.3 °C
pH:
8.04-8.38
Dissolved oxygen:
8.51-9.05 mg/L
Nominal and measured concentrations:
Nominal concentrations: 0 (control), 3.9, 7.8, 15.6, 31.3, 62.5, 125, 250 and 500 mg/L
Details on test conditions:
- Photoperiod: 16h:8h day:night
Reference substance (positive control):
not specified

Results and discussion

Effect concentrations
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
85.44 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: 95% C.L.: 72.3-101.0 mg/L

Any other information on results incl. tables

Concentration (mg/L)

Mobile animals after:

0h

3h

6h

24h

48h

3.906

20

20

20

20

20

7.81

20

20

20

20

20

15.6

20

20

20

20

20

31.3

20

20

20

20

20

62.5

20

20

19

19

16

125

20

19

19

17

3

250

20

12

1

0

0

500

20

0

0

0

0

0

20

20

20

20

20

Applicant's summary and conclusion