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EC number: 213-116-3 | CAS number: 925-21-3
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
Description of key information
The effect of the test item on the growth of Pseudokirchneriella subcapitata has been investigated over a 72-Hour period and gave the following results:
Response EC50 (mg/L) 95% Confidence Limits No Observed Effect Concentration Lowest Observed Effect Concentration
Variable.........................................................(mg/L).....................................(NOEC) (mg/L).......................................(LOEC) (mg/L)
Growth Rate.............31...............................30 - 33.................................................25...........................................................50
Yield..........................19...............................17 - 22...............................................6.25........................................................12.5
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 31 mg/L
- EC10 or NOEC for freshwater algae:
- 25 mg/L
Additional information
Introduction
A study was performed to assess the effect of the test item on the growth of the green alga Pseudokirchneriella subcapitata. The method followed was designed to be compatible with the OECD Guidelines for Testing of Chemicals (2006) No 201, "Freshwater Alga and Cyanobacteria, Growth Inhibition Test" referenced as Method C.3 of Commission Regulation (EC) No 761/2009.
Methods
Following a preliminary range-finding test, Pseudokirchneriella subcapitata was exposed to an aqueous solution of the test item at concentrations of 6.25, 12.5, 25, 50 and 100 mg/L (three replicate flasks per concentration) for 72 hours, under constant illumination and shaking at a temperature of 24 ± 1 °C. Samples of the algal populations were removed daily and cell concentrations determined for each control and treatment group, using a Coulter® Multisizer Particle Counter.
Results
Exposure of Pseudokirchneriella subcapitata to the test item gave the following results:
Response Variable |
EC50 (mg/L) |
95% Confidence Limits (mg/L) |
No Observed Effect Concentration (NOEC) (mg/L) |
Lowest Observed Effect Concentration (LOEC) (mg/L) |
Growth Rate |
31 |
30 – 33 |
25 |
50 |
Yield |
19 |
17 – 22 |
6.25 |
12.5 |
Analysis of the test preparations at 0 and 72 hours showed measured test concentrations to range from 99% to 103% of nominal and so the results are based on nominal test concentrations only.
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