Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 448-300-4 | CAS number: 88642-03-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)
- GLP compliance:
- yes
- Details on sampling:
- - Concentrations: control, saturated solution
- Sampling method: Samples were taken at the start and the end of exposure - Vehicle:
- yes
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method:Following the recommendations of OECD guidance document no. 23 (2000)6, a column elution system as generator system was applied. Once for the test a HPLC column (length 25 cm x i.d. 4.6 mm = volume 4.2 cm3 ) was prepared prior to test start. For the preparation of the column 7.8 g (5 cm3) of quartz sand (washed twice with aceton) were spiked with 15 mg of the test item. The optimal ratio of the test item stock solution to quartz sand is 0.24 mL / 1 g. The test item (401 mg were weighed out) was dissolved in 50 mL acetone and mixed thoroughly with the quartz sand in a glass petri dish. After complete evaporation of the solvent, the dry quartz sand was filled in the HPLC column. Lower homologues and impurities were purged by rinsing the column with 200 mL of dilution water. For preparation of the saturated solution the generator column was rinsed with approx. 3 L of dilution water in a circuit within 96 hours by a HPLC-pump with a flow rate of 1.2 mL/min. - Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISM
- Common name: Green alga
- Strain: SAG 86.81
- Source: Sammlung von Algenkulturen (SAG), Pflanzenphysiologisches Institut der Universität Göttingen, Göttingen, Germany
- Age of inoculum (at test initiation): four days
- Method of cultivation: Fresh stocks were prepared every month an Z-Agar. Light intensity amounted 35-70 µE*m^-2* s^-1 for 24 h per day. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 72 h
- Test temperature:
- 21-22 °C
- pH:
- 7.9 - 9.7
- Nominal and measured concentrations:
- nominal: 100% saturated solution
initial measured concentrations: 912 µg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: sterile headspace flasks with aluminium tops with PTFE seals
- Type: closed
- Headspace, fill volume: no headspace, 119 mL
- Aeration: no
- Initial cells density: 5601 cells/mL
- Control end cells density: 361131 cells/mL
- No. of vessels per concentration (replicates): 6
- No. of vessels per control (replicates): 6
GROWTH MEDIUM
- Standard medium used: yes
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: According to the guideline
- Intervals of water quality measurement: The pH-value at the beginning of the test was measured out of one additional replicate of the limit concentration and control. At the end it was measured from one pool per concentration of all replicates.
OTHER TEST CONDITIONS
- Light measurememts: Light intensity was measured prior to test start.
- Photoperiod: 24 h/d light
- Light intensity and quality: 76 - 87 µE • m-2 • s-1
EFFECT PARAMETERS MEASURED:
- Microscopic evaluation of the cells at the start and at the end of the incubation was determined. Cells were checked for any unusual cell shapes, colour differences, chloroplast morphology, flocculation, adherence of algae to test containers or aggregation of alga cells
- Chlorophyll measurement: Cell density was measured via Chlorophyll-a-fluorescence, excitation at 435 nm after 0, 24, 48, 72, 96 h
TEST CONCENTRATIONS
- Range finding study: yes
- Test concentrations: saturated solution, 1:10
- Results used to determine the conditions for the definitive study: no toxic effects were found in the saturated solution - Reference substance (positive control):
- yes
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 0.912 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- > 0.912 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 0.912 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- > 0.912 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Details on results:
- - Exponential growth in the control (for algal test): yes
- Observation of abnormalities (for algal test): none - Results with reference substance (positive control):
- - Determined over a period of 72 h from September 10 to 13, 2007
- ErC50 = 1.01 mg/L
- EyC50 = 0.42 mg/L - Validity criteria fulfilled:
- yes
- Conclusions:
- In this study the test item did not cause inhibitory effects on growth rate of algae after 72 h up to the solubility limit (saturation solution level under test conditions: 912 µg/L as inital mean measured concentration)
Reference
Table 1: Test item concentrations
Sampling date |
2008-01-22 |
2008-01-25 |
|
Start of analysis |
2008-01-22 |
2008-01-25 |
|
Saturated solution |
Test item |
||
Meas. |
Meas. |
RR [%] |
|
1:1 without algae |
0.912 |
0.890 |
98 |
1:1 with algae |
Not measured |
0.423 |
46 |
Control |
< LOQ |
< LOQ |
— |
Meas. conc. = Measured concentration, dilution factor taken in account, mean value of 2 injections
RR = Recovery rate related to the initially measured concentration
LOQ = Limit of quantification
Description of key information
The 72 -hour ErC50 and ErC10 for Pseudokirchneriella subcapitata > 912 µg/L (solubility limit).
Key value for chemical safety assessment
Additional information
One experimental algae study according to OECD 201 and GLP is available. This limit test was conducted under static conditions in a closed system with no headspace. Pseudokirchneriella subcapitata cells were exposed to saturated solution of the test item and the control. In this study the test item did not cause inhibitory effects on growth rate of algae after 72 h up to the solubility limit (saturation solution level under test conditions: 912 µg/L as inital mean measured concentration).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.