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Diss Factsheets
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EC number: 448-300-4 | CAS number: 88642-03-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Bioaccumulation: aquatic / sediment
Administrative data
Link to relevant study record(s)
- Endpoint:
- bioaccumulation in aquatic species: fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.13 (Bioconcentration: Flow-through fish test)
- GLP compliance:
- yes (incl. QA statement)
- Radiolabelling:
- no
- Details on sampling:
- - Sampling intervals/frequency for test organisms:
- Uptake phase: after 5, 24, 48, 72, 96, 168 h
- Depuration phase: 192, 216, 240, 264 h
- Sampling intervals/frequency for test medium samples:
- Uptake phase: after 5, 24, 48, 72, 96, 168 h
- Depuration phase: 192, 216, 240, 264 h - Vehicle:
- no
- Details on preparation of test solutions, spiked fish food or sediment:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: To produce the stock solution (0.2 mg/L), the test substance was weighed into water and treated for 5 minutes at 8000 rpm. with an ultra-turrax. The stock solution and synthetic fresh water were pumped into a mixing chamber. From this mixing chamber, the test solution was directly introduced into the test vessel (nominal test concentration 7 µg/L).
- Controls: synthetic fresh water in accordance with ISO 7346 - Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name: zebra fish
- Source: Bio International B.V, The Netherlands
- Date of birth: 17 December 1998
- Length at study initiation (range): 2.5 - 3.5 cm
- Weight at study initiation (mean): control: 0.36 g; test concentration: 0.35 g
- Weight at termination (mean): control: 0.30 g; test concentration: 0.34 g
- Lipid content at test initiation: 4.2% of wet weight
- Health status: Mortality while kept < 5 % ; no medical treatment
- Feeding during test
- Food type: T etra Min fish food
- Amount: 2 % of body weight per day
- Frequency: applicated in 2 portions daily - Route of exposure:
- aqueous
- Test type:
- flow-through
- Water / sediment media type:
- natural water: freshwater
- Total exposure / uptake duration:
- 174 h
- Total depuration duration:
- 89 h
- Hardness:
- 12.1 °dH
- Test temperature:
- Acclimation phase: 22.1 - 23.0 °C
Uptake phase: 21.9 - 24.2 °C
Depuration phase: 21.5 - 23.7 °C - pH:
- Acclimation phase: 7.9 - 8.1
Uptake phase: 7.6 - 7.8
Depuration phase: 7.8 - 8.1 - Dissolved oxygen:
- Acclimation phase: 7.4 - 7.7 mg/L
Uptake phase: 7.2 - 8.2 mg/L
Depuration phase: 8.0 - 9.0 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: aquarium
- Type: open
- Size, fill volume: 600 x 295 x 290 mm, 40 L
- Aeration: yes
- Renewal rate of test solution (frequency/flow rate): f requency of water renewal: about 10 times per day
- No. of organisms per vessel: 100
- No. of vessels per concentration (replicates): 1
- No. of vessels per control / vehicle control (replicates): 1
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Synthetic fresh water in accordance with ISO 7346
OTHER TEST CONDITIONS
- Photoperiod: 16 hours light / 8 hours dark - Nominal and measured concentrations:
- Nomial concentrations: control, 7 µg/L
- Reference substance (positive control):
- no
- Lipid content:
- 4.2 other: % w.w
- Time point:
- other: start of the uptake phase
- Lipid content:
- >= 6.1 - <= 6.9 other: % w.w.
- Time point:
- other: end of the depuration phase
- Key result
- Conc. / dose:
- 7 µg/L
- Type:
- BCF
- Value:
- 244 L/kg
- Basis:
- whole body w.w.
- Calculation basis:
- steady state
- Key result
- Rate constant:
- overall depuration rate constant (d-1)
- Value:
- -0.035
- Details on kinetic parameters:
- - Uptake rate constant k(s):
The rapid achievement of steady-state conditions during the uptake phase did not allow for a determination of the uptake rate constant.
- Depuration rate constant k(e): After turning to clean water conditions, the measured concentrations in fish fell down to the analytical detection limit within three days approximately. - Details on results:
- - Mortality of test organisms:
none
- Behavioural abnormalities: none
- Mortality and/or behavioural abnormalities of control: none - Validity criteria fulfilled:
- not specified
- Conclusions:
- The determined BCF of 244 L/kg indicates a low potential of the test substance to bioaccumulate.
- Endpoint:
- bioaccumulation in aquatic species: fish
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Justification for type of information:
- See read-across justification attached to section 13.
- Reason / purpose for cross-reference:
- read-across source
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.13 (Bioconcentration: Flow-through fish test)
- Key result
- Conc. / dose:
- 7 µg/L
- Type:
- BCF
- Value:
- 244 L/kg
- Basis:
- whole body w.w.
- Calculation basis:
- steady state
Referenceopen allclose all
Description of key information
BCF = 244 L/kg for Danio rerio (EU method C. 13)
Key value for chemical safety assessment
- BCF (aquatic species):
- 244 L/kg ww
Additional information
For the test substance no bioaccumulation study is available. However, the bioaccumulation potential of a suitable surrogate substance in zebra fish Danio rerio was investigated using a flow through system set up according to the EU method C. 13. The fish were exposed via water to the test substance for 174 hours. After a depuration period of 89 hours the test was terminated and a mean growth and lipid corrected BCF of 244 L/kg ww based on the whole body wet weight was determined.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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