Oleic acid, monoester with oxybis(propanediol)

Endpoint summary

Additional information

There are no data available for the short-term toxicity to fish and the long-term toxicity to aquatic invertebrates of Oleic acid, monoester with oxybis(propandiol) (CAS 49553-76-6). In order to fulfil the standard information requirements set out in Annex VIII, 9.1.3 and Annex IX, 9.1.5, respectively, in accordance with Annex XI, 1.5, of Regulation (EC) No 1907/2006, read-across from one structurally related substance was conducted.

In accordance with Article 13 (1) of Regulation (EC) No 1907/2006, "information on intrinsic properties of substances may be generated by means other than tests, provided that the conditions set out in Annex XI are met.” In particular for human toxicity, information shall be generated whenever possible by means other than vertebrate animal tests, which includes the use of information from structurally related substances (grouping or read-across).

Having regard to the general rules for grouping of substances and read-across approach laid down in Annex XI, Item 1.5, of Regulation (EC) No 1907/2006 whereby substances may be predicted as similar provided that their physicochemical, toxicological and ecotoxicological properties are likely to be similar or follow a regular pattern as a result of structural similarity.

Ecotoxicological parameters for the aquatic toxicity of the target substance Oleic acid, monoester with oxybis(propandiol) and the source substance 2,3-dihydroxypropyl oleate are presented in the following table.

CAS	49553-76-6	111-03-5
Chemical Name	Oleic acid, monoester with oxybis(propandiol)	2,3-dihydroxypropyl oleate
MW	430	356.54
Short-term toxicity to fish	RA: CAS 111-03-5	LC50 (96 h): >102.8 mg/L (measured)
Long-term toxicity to fish	Waiving based on CSA	--
Short-term toxicity to aquatic invertebrates	EC50 (48 h): 37.7 mg/L (meas.)	EC50 (48 h): 49 mg/L
Long-term toxicity to aquatic invertebrates	RA: CAS 111-03-5	NOEC: 1.85 mg/L (measured)
Toxicity to aquatic algae	NOEC (72 h): 0.0113 mg/L (meas.)	NOEC (72 h): 0.40 mg/L
Toxicity to microorganisms	NOEC (14d) ≥ 36,67 mg/L (Tox-control)	--

 

The above mentioned substances are considered to be similar on the basis of the structural similar properties and/or activities. The available endpoint information is used to predict the same endpoints for Oleic acid, monoester with oxybis(propandiol).

A detailed analogue approach justification is provided in the technical dossier (see IUCLID Section 13).

In accordance with Regulation (EC) No. 1907/2006, Annex XI, 1.5, grouping and read-across, the target substance Oleic acid, monoester with oxybis(propandiol) (CAS 49553-76-6) and the source substance 2,3-dihydroxypropyl oleate (CAS 111-03-5) show similar toxicity profile to aquatic organisms based on the results from short-term studies with fish, aquatic invertebrates and algae and long-term results on toxicity to aquatic invertebrates. Both substances are structurally similar. They are both esters with oleic acid as main fatty acid component.The target substance is an ester consisting in addition on mono-esters of diglycerol in comparison to monoglycerol for the source substance. Thus, the source substance 2,3-dihydroxypropyl oleate is a very close analogue to the target substance Oleic acid, monoester with oxybis(propandiol).

The data gaps for short-term toxicity to fish and long-term toxicity to aquatic invertebrates were adequately filled, since both substances are characterized by a similar pattern in the ecotoxicological profile. Effects were observed for short-term daphnia as well as for toxicity to algae for both substances in the same range (see table above) with algae being the most sensitive organism. For the source substance 2,3-dihydroxypropyl oleate no toxicity was observed for short-term toxicity to fish up to the limit of water solubility (LC50 (96h) > 102.8 mg/L). As the target and the source substance are structurally similar and show a similar ecotoxicological profile for daphnia and algae, it is expected that fish is also for the target substance the species showing the least sensitivity. Therefore, the data gap for the target substance for short-term toxicity to fish can be covered with the data from the source substance 2,3-dihydroxypropyl oleate.

As the target and the source substance show a similar ecotoxicological profile for short-term toxicity to aquatic invertebrates and toxicity to algae, similar toxicity will be expected for long-term toxicity to aquatic invertebrates as well. Thus for the target substance, aquatic invertebrates are not expected to be more sensitive than algae. Therefore, the data gap for the target substance for long-term toxicity to aquatic invertebrates can be covered with the data from the source substance 2,3-dihydroxypropyl oleate.

Based on the results from a toxicity control included in the biodegradation study (OECD 301B) it can be concluded that Oleic acid, monoester with oxybis(propandiol) (CAS 49553-76-6) is not expected to exhibit effects on the microorganism community in sewage treatment plants and inhibition of the subsequent degradation process is not expected (NOEC (21d) of ≥ 36.67 mg/L).

In conclusion, the target substance Oleic acid, monoester with oxybis(propandiol) (CAS 49553-76-6) shows toxicity to aquatic life with long lasting effects. Therefore, Oleic acid, monoester with oxybis(propandiol) is classified in accordance with the 2nd ATP of Regulation (EC) No. 1272/2008 (CLP) as long-term aquatic hazard Chronic Category 2. As it can be seen in the data matrix of the analogue justification in IUCLID Section 13, all reliable data from the target and the source substance support the read-across for the endpoint short-term toxicity to fish and the long-term toxicity to aquatic invertebrates by showing a consistent pattern of results.