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EC number: 608-211-5 | CAS number: 284462-37-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 10/2004
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- Deviations:
- no
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- - Loaded samples and Control: at 0, 24, 48, 72 and 96 hours
- Sampling method: from freshly prepared medium
- Routinely, the samples are analysed immediately. Only in exceptional cases, they are stored overnight deeply frozen and protected from light.
- Observations on mortality at 2, 24, 48, 72 and 96 h - Vehicle:
- no
- Details on test solutions:
- PRETREATMENT OF TEST ITEM:
A stock solution was prepared to give the desired series of test concentrations. To achieve this 1000.5 mg of the test item were added to 10 litres of dilution water, treated for 1 h in an ultrasonic bath and afterwards stirred for 24 h on a magnetic stirrer. - Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- - supplier: Aqua KlöGer (Germany)
- mean standard length (n=14): 3.16 cm (S.D. = 0.23 cm)
acclimatisation: - temperature: 20 - 24 °C; - dissolved oxygen: > 5 mg/l; - medication: none; - mortalities during acclimatization: < 5 % ; - feeding: Commercial fish food, daily. Feeding discontinued 24 h prior to test start; - Stock held since 2004-07-17 and acclimatised to the test conditions since then - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Hardness:
- 15.2 °dH (= 271.3 mg/l CaCO3)
- Test temperature:
- 22.7 - 23.5 °C (daily monitoring)
- pH:
- 7.6 - 7.9 (daily monitoring)
- Dissolved oxygen:
- 7.8 - 8.6 (daily monitoring)
- Nominal and measured concentrations:
- nominal concentration: 25, 50 and 100 mg/L
- Details on test conditions:
- TEST SYSTEM:
- keeping water: synthetic fresh water according to ISO 7346
- glass aquaria holding 5 L of test media
- experimental design: 3 test concentrations plus 1 control; 10 animals per test concentration; no feeding during the exposure period; static system
- photoperiod: 16 hours light, 8 hours dark
- temperature: 20 - 24 °C +/- 1 °C
- aeration: gentle aeration via narrow glass tubes
- method of administration: stock solution
- solubilizer or other auxiliaries: ultrasonic bath, magnetic stirrer
- test concentration: 25, 50 and 100 mg/L plus one control
- Method of initiation: animals were placed in prepared media
- test parameter: mortality (recorded daily)
- Loading: 0.65 g body weight (wet weight) per litre
- medium renewal: none - Reference substance (positive control):
- not required
- Duration:
- 96 h
- Dose descriptor:
- LC100
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 90.2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC0
- Effect conc.:
- 50 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- All results are expressed in terms of nominal concentrations. Recovery rates ranges from 103.6 - 105.2% of nominal values at 0 hour, from 100.7 - 104.1% of nominal values at 24 hours, from 98.5 - 102.6% of nominal values at 48 hours and from 96.9 - 102.3% of nominal values at 72 hours and at 96 hours, respectively.
- Validity criteria fulfilled:
- yes
- Remarks:
- (-The mortality in the controls <10 % by the end of test. -The dissolved oxygen conc > 60%of the air-saturation value during study. -The pH did not vary by more than 1 unit.- substance concenteration > 80% during test )
- Conclusions:
- After 96 hours a LC 50 of 90.2 mg/L and a LC 100 of > 100 mg/L against fish was observed.
- Executive summary:
In order to test acute toxicity to fish of the substance, Danio rerio was exposed to the test solution of 3 nominal concentration of the test substance ( 25, 50 and 100 mg/L) and blank control solution for a period of 96 h under static conditions.
Mortality and visible abnormalities were recorded at 2, 24, 48, 72 and 96 h. A LC 100 of > 100 mg/L and LC50 of 90.2 mg/L was observed after an exposure of 96h. This toxicity study is classified as acceptable and satisfies the guideline requirements for the toxicity study to fish (Danio rerio).
Reference
Description of key information
After 96 hours a LC 50 of 90.2 mg/L and a LC 100 of > 100 mg/L against fish was observed.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Dose descriptor:
- LC50
- Effect concentration:
- 90.2 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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