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EC number: 683-287-0 | CAS number: 877670-90-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Skin sensitisation
Administrative data
- Endpoint:
- skin sensitisation: in vivo (LLNA)
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2008
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP study done according to guideline
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 008
- Report date:
- 2008
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 429 (Skin Sensitisation: Local Lymph Node Assay)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Type of study:
- mouse local lymph node assay (LLNA)
Test material
- Reference substance name:
- 2-(2-{1-[(2-methoxyphenyl)carbamoyl]-2-oxopropyl}diazen-1-yl)benzoic acid
- EC Number:
- 683-287-0
- Cas Number:
- 877670-90-1
- Molecular formula:
- C18H17N3O5
- IUPAC Name:
- 2-(2-{1-[(2-methoxyphenyl)carbamoyl]-2-oxopropyl}diazen-1-yl)benzoic acid
- Details on test material:
- Batch 939-76
Constituent 1
In vivo test system
Test animals
- Species:
- mouse
- Strain:
- CBA
- Sex:
- female
Study design: in vivo (LLNA)
- Vehicle:
- other: N,N-Dimethylformamide (DMF).
- Concentration:
- 2.5, 1, 0.5% W/V in DMF
- No. of animals per dose:
- 4
- Positive control substance(s):
- hexyl cinnamic aldehyde (CAS No 101-86-0)
Results and discussion
In vivo (LLNA)
Resultsopen allclose all
- Parameter:
- SI
- Remarks on result:
- other: Test Group Name Negative control (solvent) DMF 1.0 Anthranilic Acid-AAOA, 2.5% 2.1 Anthranilic Acid-AAOA, 1.0% 1.5 Anthranilic Acid-AAOA, 0.5% 1.2
- Parameter:
- other: disintegrations per minute (DPM)
- Remarks on result:
- other: Test Group Name Negative control (solvent) DMF 860.1 Anthranilic Acid-AAOA, 2.5% 1842.1 Anthranilic Acid-AAOA, 1.0% 1254.5 Anthranilic Acid-AAOA, 0.5% 1052.9
Any other information on results incl. tables
See Figure 1: Test Item Stimulation Index Values
Applicant's summary and conclusion
- Interpretation of results:
- not sensitising
- Remarks:
- Migrated information
- Conclusions:
- Under the conditions of the present assay Anthranilic Acid-AAOA tested up to the maximum feasible concentration (2.5 %) in an appropriate solvent was shown to have no sensitisation potential (non-sensitiser) in the Local Lymph Node Assay, because no SI value equal to or greater than the threshold value of 3.0 was observed.
- Executive summary:
The aim of this study was to determine the skin sensitisation potential of Anthranilic Acid-AAOA following dermal exposure. The maximum concentration of test item in an appropriate solvent was established according to OECD guideline 429. The maximum available concentration based on solubility was 2.5 % (% w/v) in N,N-Dimethylformamide (DMF). A preliminary irritation/toxicity test was performed showing that the maximum concentration of test item of 2.5 % in DMF was acceptable. Sixteen female CBA/Ca mice were allocated to four groups of four animals: - three groups received test item at concentrations of 2.5 %, 1.0 % and 0.5 %, - the negative control group received the solvent (DMF). Each substance was applied on the external surface of each ear (25 ul/ear) of the animals for three consecutive days (Day 1, 2 and 3). There was no treatment on Day 4, 5 and 6. On Day 6, the cell proliferation in the local lymph nodes was measured by incorporation of tritiated methyl thymidine (3HTdR) and the obtained values were used to calculate stimulation indices (SI). No mortality or systemic clinical signs were observed during the study. No treatment related effects were observed on animal body weights in any treatment groups. No cutaneous reactions were observed at the treated site in any of the treated groups. No significant lymphoproliferative response (SI greater than or equal to 3) was observed with Anthranilic Acid-AAOA at the applied concentrations. The stimulation index values (SI) were 2.1, 1.5 and 1.2 at concentrations of 2.5 %, 1.0 % and 0.5 %, respectively. The stimulation index values corresponded to the conventional biological dose response. The result of the latest reliability check (performed within an interval of no longer than six months, Study code: 08/654-037E) was used to demonstrate the appropriate performance of the assay in accordance with the OECD guideline 429. The positive control substance alpha-Hexylcinnamaldehyde (HCA) was examined at a concentration of 25 % in the relevant vehicle. A significant lymphoproliferative response (SI greater than or equal to 3) was noted for HCA with stimulation index value of 7.1, the result confirms the validity of the LLNA in this laboratory. Conclusion: Under the conditions of the present assay Anthranilic Acid-AAOA tested in an appropriate solvent up to the maximum feasible concentration (2.5 %) was shown to have no sensitisation potential (non-sensitiser) in the Local Lymph Node Assay, because no SI value equal to or greater than the threshold value of 3.0 was observed.
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