p,p'-dinitrobibenzyl

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1990

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: well performed study according to acknowledged standard but with poor documentation

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1990

Report date:

1990

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

1,1'-ethane-1,2-diylbis(4-nitrobenzene)

EC Number:

212-001-5

Cas Number:

736-30-1

Molecular formula:

C14H12N2O4

IUPAC Name:

1,1'-ethane-1,2-diylbis(4-nitrobenzene)

Sampling and analysis

Analytical monitoring:

no

Test solutions

Details on test solutions:

Preliminary dispersion in 1% Tween 80 -acetone and dispersed in 20 litres of diluent with the aid of shielded propeller-stirrers.

Test organisms

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

TEST ORGANISM
- Common name: Rainbow trout
- Source: Trafalgar Nurseries, Downton, Salisbury, U.K.
- Length at study initiation : 4.9 +- 0.6 cm
- Weight at study initiation: 1.55 +- 0.47 g

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Test conditions

Hardness:

Approximately 50 mg/l as CaC03

Test temperature:

14 ± 1°C.

Nominal and measured concentrations:

1.0 mg/L

Details on test conditions:

Test Vessels: 20 L
Aeration: With aid of shielded propeller stirrers

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96

Dose descriptor:

LC0

Effect conc.:

1 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: saturated solution

Effect concentrations 2

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 1 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: saturated solution

Details on results:

Concentration (mg/l) Cumulative Mortality (initial population = 10)
24 hours 48 Hours 72 Hours 96 Hours
Control 0 0 0 0
1.0 0 0 0 0
1.0 0 0 0 0

Applicant's summary and conclusion

Validity criteria fulfilled:

not specified

Conclusions:

The LC0 (96 h) = 1.0 mg/L and the LC50>1.0 mg/L for the saturated solution

Executive summary:

The substance was tested for toxicity to fish (rainbow trout). Due to the low solubility in water the test was performed in replicate with a concentration of 1.0 mg/L. Even with this small concentration only a dispersion was achieved. After 96 hours no mortality was observed..