p,p'-dinitrobibenzyl

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: inherent biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1990 - 1991

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: well performed study according to acknowledged standard but with poor documentation

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1991

Report date:

1991

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 302 C (Inherent Biodegradability: Modified MITI Test (II))

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

1,1'-ethane-1,2-diylbis(4-nitrobenzene)

EC Number:

212-001-5

Cas Number:

736-30-1

Molecular formula:

C14H12N2O4

IUPAC Name:

1,1'-ethane-1,2-diylbis(4-nitrobenzene)

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge (adaptation not specified)

Details on inoculum:

A mixed population of activated sewage sludge microorganisms.
Source: The aeration stage of Severn Trent PLC Sewage Treatment Plant, Belper, Derbyshire.
Preparation: The activated sludge was maintained at a temperature of 21 ± 1°C for 24 hours. The sluge was washed and settled
repeatedly using dilution water prior to use.
Usage Rate: Equivalent to 100 mg dry weight/l.

Duration of test (contact time):

28 d

Initial test substance concentration

Initial conc.:

30 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

O2 consumption

Reference substance

Reference substance:

aniline

Remarks:

100 mg/L

Results and discussion

% Degradation

% Degradation 1

Parameter:

% degradation (O2 consumption)

Value:

0

Sampling time:

10 d

% Degradation 2

Parameter:

% degradation (O2 consumption)

Value:

7

Sampling time:

18 d

% Degradation 3

Parameter:

% degradation (O2 consumption)

Value:

0

Sampling time:

28 d

BOD5 / COD results

Results with reference substance:

Aniline attained 56% biodegradation after 7 days and 70% after 14 days thereby
confirming the suitability of the inoculum and culture conditions. Total Organic
Carbon (TOC) analysis showed that aniline attained 94% degradation after 28
days.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

not inherently biodegradable

Conclusions:

The substance is not inherently biodegradable

Executive summary:

The substance was tested for inherent biodegradability with the modified MITI (II) - test. It attained negligible degradation within 28 days calculated from oxygen uptake and therefore, cannot be considered as inherently biodegradable under the strict terms and conditions of the OECD Guidelines.