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EC number: 700-720-1
CAS number: -
Evaluation after 72 h
Statistically significant differences of
growth rates and yield compared to control values are marked (+), not
significant differences are marked (-).
Nominal test item loading
Inhibition of yield
negative inhibitions =
increase of growth
*) significance caused by
increase of growth
Table 2: TOC Content of
the Test Media at Test Start and Test End
Nominal test item loading[mg/L]
Test start (without alga), 0 h
Test end (without alga), 72 h
The toxicity of C-Wax Type OP to the unicellular freshwater green alga
Desmodesmus subspicatus was determined according to the principles of
OECD 201 and compliant to GLP (reliability category 1) at
DR.U.NOACK-LABORATORIEN in 31157 Sarstedt, Germany from March 21 to 25,
2011 with the definitive exposure phase from March 22 to 25, 2011. The
aim of the study was to assess the effects on growth rate and yield over
a period of 72 h.
The study was conducted under static conditions with an initial cell
density of 4 x 10 E(3) cells/mL. Based on a preliminary range finding
test, a limit test with a water accommodated fraction (WAF)* was carried
out. The water accommodated fraction was prepared with a nominal loading
of 100 mg/L test item C-Wax Type OP/L with dilution water one day prior
to application. The WAF was shaken with 20 rpm for 24 h at room
temperature. After a separation phase of 1 h the WAF was taken from the
homogeneous liquid phase. Six replicates were tested for the limit
concentration and the control. Environmental conditions were determined
to be within the acceptable limits.
The concentration of C-Wax Type OP was determined at the beginning and
end of the test via TOC-analysis at the limit loading level and control.
Due to the insolubility of the test item in water, all effect values are
given based on the nominal loading of the test item (water accomodated
fraction according to OECD Guidance Document, No. 23 ):
NOEC (72h, growth rate and yield) = 100 mg/L;
EC10 (72h, growth rate and yield) > 100 mg/L;
EC50 (72h, growth rate and yield) >100 mg/L.
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