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EC number: 258-292-2 | CAS number: 53012-41-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 15.11.1999 - 06.12.1999
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: The study was conducted in GLP compliance and in accordance with internationally acknowledged guidelines for testing of chemicals.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 999
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 850.1010 (Aquatic Invertebrate Acute Toxicity Test, Freshwater Daphnids)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 1-(2,6-dimethylphenoxy)acetone
- EC Number:
- 258-292-2
- EC Name:
- 1-(2,6-dimethylphenoxy)acetone
- Cas Number:
- 53012-41-2
- Molecular formula:
- C11H14O2
- IUPAC Name:
- 1-(2,6-dimethylphenoxy)propan-2-one
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Strain: Daphnia magna (Straus ), clone 5
- Sex: female
- Source: supplied 1997 by the Umweltbundesamt, Institut für Wasser-, Boden- und Lufthygiene, Berlin, Germany
- Age at study initiation (mean and range, SD): 6.5 - 22 hours
- Method of breeding: The Daphnia were bred in the laboratories of IBACON under similar temperature and light conditions as in the test, and in reconstituted water of a similar quality regarding to pH, components of the main ions and total hardness as the test water used in the test (see below). The test organisms were not first brood progeny.
ACCLIMATION
- Acclimation period: for 6.5 hours under test conditions
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Post exposure observation period:
- none
Test conditions
- Hardness:
- 2.5 mmol/L (= 250.0 mg/L) as CaC03
- Test temperature:
- 21°C
- pH:
- pH 7.7 to 7.8
- Dissolved oxygen:
- at least 8.1 mg/L or higher
- Nominal and measured concentrations:
- 4.6, 10, 21, 46 and 100 mg test substance/L (nominal)
- Details on test conditions:
- TEST SYSTEM
- Test vessel: glass beakers of 100 mL volume with 50 mL testmedium
- Aeration:
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2
TEST MEDIUM / WATER PARAMETERS
In deionized water (conductivity < 5 µScm-1 ) analytical grade salts were added to following nominal concentrations:
CaCl2 x 2H20 2.0 mmol/L (= 294.0 mg/L)
MgS04 x 7H20 0.5 mmol/L (= 123.0 mg!L)
NaHC03 0.75 mmol/L (= 65.0 mg/L)
KCl 0.075 mmol/L (= 5.8 mg!L)
Water Hardness: 2.5 mmol/L (= 250.0 mg/L) as CaC03
Alkalinity: 0.8 mmol/L
ratio of Ca : Mg = 4: 1 (based on molarity)
Na : K = 10: 1 (based on molarity)
OTHER TEST CONDITIONS
- Photoperiod: 16 h light: 8 h dark
- Light intensity: approximately 600 lux
- Surrounding Type: controlled environment room
TEST CONCENTRATIONS
- Range finding study: yes
- Test concentrations: 4.6, 10, 21, 46 and 100 mg test substance/L - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 10 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: immobility or mortality
- Remarks on result:
- other: Those animals not able to swim within 15 seconds after gentle agitation of the test beaker were considered to be immobile.
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 53.4 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: immobility or mortality
- Remarks on result:
- other: with 95 % eonfidence limits from 35.9 to 79.4 mg/L. Those animals not able to swim within 15 seconds after gentle agitation of the test beaker were considered to be immobile
- Duration:
- 48 h
- Dose descriptor:
- EC100
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: mortality or immobilization
- Remarks on result:
- other: Those animals not able to swim within 15 seconds after gentle agitation of the test beaker were considered to be immobile
- Duration:
- 48 h
- Dose descriptor:
- EC0
- Effect conc.:
- 10 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: mortality or immobilization
- Remarks on result:
- other: Those animals not able to swim within 15 seconds after gentle agitation of the test beaker were considered to be immobile
- Details on results:
- Results after 24 hours: The 24-hour EC 50 and EC 100 of the test substanee were higher than 100 mg test substanee/L.
The 24-hour EC 0 was determined to be 46 mg test substanee/L. - Results with reference substance (positive control):
- For the evaluation of the quality of the Daphnia clone and the experimental conditions the substance potassium dichromate p.A. (E. Merck, Darmstadt, Germany) is tested at least twice a year to demonstrate satisfactory test conditions.
- Reported statistics and error estimates:
- The 24-hour EC 50 and the 95 % confidence value could not be calculated due to the low toxic effect after 24 hours test duration. The 48-hour EC 50 was calculated by the Moving average interpolation. The NOEC, EC 0 and EC 100 were determined directly from the raw data.
Any other information on results incl. tables
Influence of 2,6-DMPA on the mobility of Daphnia magna
Concentration of the test substance | No. of Daphnia tested | No. of immobilized Daphnia after | % of immobilized Daphnia after | ||
[mg/L] | 24h | 48 h | 24h | 48h | |
control | 20 | 0 | 0 | 0 | 0 |
4.6 | 20 | 0 | 0 | 0 | 0 |
10 | 20 | 0 | 0 | 0 | 0 |
21 | 20 | 0 | 1 | 0 | 5 |
46 | 20 | 0* | 9** | 0 | 45 |
100 | 20 | 14* | 17** | 70 | 85 |
* a1l surviving Daphnia looked pale
** a1l surviving Daphnia showed mobilization only after gentle agitation of the test beakers
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 48-hour EC 50 was calculated tobe 53.4 mg test substance/L with 95 % eonfidence limits from 35.9 to 79.4 mg/L. The 48-hour EC 0 and the 48-hour NOEC (highest eoneentration tested without toxie effects after 48 hours) of 2,6-DMPA were deterrrrined tobe 10 mg test substance/L, since no significant immobilization rate and no other signs of intoxication were observed at the test animals up to and including this test coneentration. The 48- hour EC 100 was high er than 100 mg test substance/L.
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