2-methylbut-3-yn-2-ol

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Non-GLP study.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Report date:

1976

Materials and methods

Test guideline

Test guideline 1

Qualifier:

equivalent or similar to guideline

Guideline:

EU Method B.1 (Acute Toxicity (Oral))

Deviations:

not specified

Test guideline 2

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 401 (Acute Oral Toxicity)

Deviations:

not specified

GLP compliance:

no

Test type:

acute toxic class method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

2-methylbut-3-yn-2-ol

EC Number:

204-070-5

EC Name:

2-methylbut-3-yn-2-ol

Cas Number:

115-19-5

Molecular formula:

C5H8O

IUPAC Name:

2-methylbut-3-yn-2-ol

Details on test material:

- Name of test material (as cited in study report): Methylbutinol

Test animals

Species:

rat

Strain:

other: Albino

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source:
- Age at study initiation: Young
- Weight at study initiation: 200-300 grams
- Fasting period before study: 24 hours
- Housing: common cage
- Diet: ad libitum
- Water: ad libitum

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

unchanged (no vehicle)

Details on oral exposure:

MAXIMUM DOSE VOLUME APPLIED: 4000 mg/kg bw

Doses:

Male/female: 500, 1000, 1250, 1600, 2000, 3200 and 4000 mg/kg bw

No. of animals per sex per dose:

5 male, 5 female per dose

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations: daily
- Necropsy of survivors performed: no
- Other examinations performed: clinical signs, body weight,organ weights, histopathology, other: no

Results and discussion

Effect levels

Effect levels 1

Sex:

male

Dose descriptor:

LD0

Effect level:

1 000 mg/kg bw

Based on:

test mat.

Effect levels 2

Sex:

male

Dose descriptor:

LD50

Effect level:

1 410 mg/kg bw

Based on:

test mat.

95% CL:

1.06 - 1.88

Effect levels 3

Sex:

male

Dose descriptor:

LD100

Effect level:

2 500 mg/kg bw

Based on:

test mat.

Effect levels 4

Sex:

female

Dose descriptor:

LD0

Effect level:

500 mg/kg bw

Based on:

test mat.

Effect levels 5

Sex:

female

Dose descriptor:

LD50

Effect level:

1 620 mg/kg bw

Based on:

test mat.

95% CL:

1.32 - 2

Effect levels 6

Sex:

female

Dose descriptor:

LD100

Effect level:

2 500 mg/kg bw

Based on:

test mat.

Mortality:

Males
Group Dose level (mg/kg bw) Survivors Deaths
I 500 5 0
II 1000 5 0
III 1250 3 2
IV 1600 2 3
V 2000 1 4
VI 2500 0 5
VII 3200 0 5
VIII 4000 0 5

Females
Group Dose level (mg/kg bw) Survivors Deaths
I 0.5 5 0
II 1 4 1
III 1 4 1
IV 1.6 3 2
V 2 1 4
VI 2.5 0 5
VII 3.2 0 5
VIII 4 0 5

Clinical signs:

other: Males: The animals dosed at 500 mg/kg bw and 1000 mg/kg bw were sluggish with unkempt coats for 36 - 48 hours. Staggering gait, impaired locomotion, labored breathing, and lethargy were noted after intubation at levels ranging from 1250 mg/kg bw to 2500 m

Applicant's summary and conclusion

Interpretation of results:

harmful

Remarks:

Migrated information Criteria used for interpretation of results: EU

Conclusions:

The acute toxicity study revealed LD50 values of 1410 mg/kg bw for males and 1620 mg/kg bw for females.

Executive summary:

An acute oral toxicity study was carried out according to a method similar or equivalent to EU method B.1 and OECD guideline 401. Male and female rats were administered doses of 500, 1000, 1250, 1600, 2000, 3200 and 4000 mg/kg bw per oral gavage. The resulting LD50 value for males was 1410 mg/kg bw and the LD50 value for females was 1620 mg/kg bw.