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EC number: 218-491-7 | CAS number: 2163-00-0
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
Description of key information
1,6-Dichlorohexane is classified as not readily biodegradable in the 10-d-window and after 61 days according to OECD guideline no. 310.
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
Additional information
In the key study, 1,6-dichlorohexane was tested in a 61 d biodegradation study (2012 -0242 -DGO) according to OECD guideline no. 310.The ready biodegradability of the test item 1,6-dichlorohexane was determined with a non adapted activated sludge over a test period of 61 days in the Headspace Test. The test item was tested at a concentration of 30 mg/L in triplicates (test end: 5 replicates), corresponding to a carbon content of 13.4 mg C/L. The biodegradation of the test item was followed by TIC analyses of the quantity of CO2 produced by the respiration of bacteria. The amount of TIC produced was calculated by correcting the results of the test and reference item for endogenous TIC production of the control groups and the blind value of the sodium hydroxide solution.
The test item replicates reached the 10 % level after a long lasting adaptation phase on day 31 and the biodegradation came to 54 % after 61 days. The 95 % confidence interval on day 61 was 49 - 60 %.The test item is classified as not readily biodegradable in the 10 -d-window and after 61 days.
In the first supporting study, 1,6-dichlorohexane was tested in a 7d biodegration study (BASF AG.OECD301F.1980) according to OECD guideline 301 F. In this study the test item showed the degradation of 0% (TOC removal) after 7 days.
Four further non-standard studies showed also only low degradation:
In the second supporting study (Janssen et al_1987), the substrate concentration was decreased to < 0.0005 mM (initial concentration: 0.2 mM) after 6 days.
In a further study (Omori and Alexander_1978), Pseudomonas sp. grew on and resting cells dehalogenated 1,6-dichlorohexane. Chloride release: 6 µg/mL per 48 h.
In two further studies (Heath et al_2004; Heath et al_2006), up to 5 % of chlorine was removed from 1,6-dichlorohexane.
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