4-chloro-α,α,α-trifluoro-3-nitrotoluene

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

September 13 - October 18, 1999

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1999

Report date:

1999

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

4-chloro-α,α,α-trifluoro-3-nitrotoluene

EC Number:

204-451-6

EC Name:

4-chloro-α,α,α-trifluoro-3-nitrotoluene

Cas Number:

121-17-5

Molecular formula:

C7H3ClF3NO2

IUPAC Name:

1-chloro-2-nitro-4-(trifluoromethyl)benzene

Details on test material:

Purity: 99.2%
Stability in water: maximum 7 days at room temperature
Solubility in water: Insoluble
pH in aqueous solution: 3.8 at concentration of 1/1
Aggregate state under storage conditions: Liquid
Density: 1.537 g/mL
Vapour pressure: 1.6kPa at 20°C
Storage conditions: At room temperature.

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Daphnia magna

Study design

Test type:

static

Water media type:

saltwater

Limit test:

yes

Total exposure duration:

48 h

Test conditions

Hardness:

2.5 mmol/l

Test temperature:

20-21 °C

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

19.7 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks on result:

other: 16.3-23.8

Effect concentrations 2

Duration:

24 h

Dose descriptor:

EC0

Effect conc.:

6.7 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Effect concentrations 3

Duration:

24 h

Dose descriptor:

EC100

Effect conc.:

63.6 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Effect concentrations 4

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

9.5 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks on result:

other: 6.7-13.6

Effect concentrations 5

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

6.7 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Effect concentrations 6

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

6.7 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Effect concentrations 7

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

13.6 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Any other information on results incl. tables

The mean measured test item concentrations in the analyzed test media samples were 7.2, 15.9, 32.7 and 72.5 mg/L at the start of the test. The samples from the lowest test concentration (nominal 4.6 mg/L) were not analyzed since this concentration were below the 48 -hour NOEC. The concentrations of test item decreased to values in the range from 72 to 84% of the initially measured values after the 48hour test period.

The losses might have been caused by a precipitation of the finely disperse test item, or due to a loss by absorption onto the glass surfaces. Therefore, all biological results are related to the total mean measured test item concentrations, calculated as the average over all measurements per test concentration during the test period. These were 6.7 mg/L (92% of initially measured concentration), 13.6 mg/L (86% of initial), 28.2 mg/L (86% of initial) and 63.6 mg/L (88% of initial).

Applicant's summary and conclusion

Conclusions:

48-h EC50 : 9.5 mg/l
48-h NOEC : 6.7 mg/l