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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Genetic toxicity: in vivo

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Administrative data

Endpoint:
in vivo mammalian somatic cell study: cytogenicity / erythrocyte micronucleus
Remarks:
Type of genotoxicity: chromosome aberration
Type of information:
experimental study
Adequacy of study:
other information
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
other: Test protocol and results insufficent reported for evaluation

Data source

Referenceopen allclose all

Reference Type:
publication
Title:
Study of artificial flavoring substances for mutagenicity in the Salmonella/Microsome, BASC and micronucleus tests
Author:
Wild D.K., Gocke M., and Eckhardt K.
Year:
1983
Bibliographic source:
Food Chem. Toxicol., 21, 707-719.
Reference Type:
other: Peer-review database
Title:
No information
Bibliographic source:
Toxline, [URL]: http://toxnet.nlm.nih.gov/cgi-bin/sis/htmlgen?TOXLINE, CAS n° 103-50-4, toxline abstract n° 27.

Materials and methods

Principles of method if other than guideline:
Several compounds incl. dibenzyl ether used as artifical flavouring substances were studied for mutagenic properties in a micronuleus test on mouse bone marrow.
GLP compliance:
not specified
Type of assay:
micronucleus assay

Test material

Constituent 1
Chemical structure
Reference substance name:
Dibenzyl ether
EC Number:
203-118-2
EC Name:
Dibenzyl ether
Cas Number:
103-50-4
Molecular formula:
C14H14O
IUPAC Name:
[(benzyloxy)methyl]benzene
Details on test material:
Dibenzyl ether was supplied by EGA Chemie, Steinheim

Test animals

Species:
mouse
Strain:
NMRI
Sex:
male/female
Details on test animals or test system and environmental conditions:
No data

Administration / exposure

Route of administration:
intraperitoneal
Vehicle:
No data
Details on exposure:
No data
Duration of treatment / exposure:
Mice were killed at 30 hr
Frequency of treatment:
Twice (0 and 24 hr)
Post exposure period:
None
Doses / concentrations
Remarks:
Doses / Concentrations:
0, 400, 700, or 1000 mg/Kg
Basis:
nominal conc.
No. of animals per sex per dose:
Four mice per dose
Control animals:
yes, concurrent vehicle
Positive control(s):
no data

Examinations

Tissues and cell types examined:
mouse bone marrow
Details of tissue and slide preparation:
no data
Evaluation criteria:
no data
Statistics:
no data

Results and discussion

Test results
Sex:
male/female
Genotoxicity:
negative
Toxicity:
not specified
Vehicle controls validity:
not specified
Negative controls validity:
not specified
Positive controls validity:
not specified
Additional information on results:
no data

Any other information on results incl. tables

All mice trated with 0 (vehicle), 400, 700 or 1000 mg/kg bw survived. At a dose of 0 mg/kg bw 1.8 micronuleated polychromatic erythrocytes /1000 PE, at a dose of 400 mg/kg bw 3.0 micronuleated polychromatic erythrocytes /1000 PE, at a dose of 700 mg/kg bw

3.7 micronuleated polychromatic erythrocytes /1000 PE and at a dose of 1000 mg/kg bw 2.0 micronuleated polychromatic erythrocytes /1000 PE were found.

Applicant's summary and conclusion

Conclusions:
Interpretation of results: negative
Executive summary:

Male and female NMRI mice (10 -14 wks old) were treated twice (at 0 and 24 hr) with dibenzyl ether in olive oil. In each dose group 4 mice were treated with either 0, 400, 700, or 1000 mg/kg bw). The mice were killed and bone-marrow smears were prepared 30 hr after treatment. The smears were stained and scored. The mean number of micronucleated polychromatic erytrocytes/1000 polychromatic erytrocytes were determined. Dibenzyl ether was negative in this test.