2-Isobutyryl-3,N-diphenylacrylamide

Toxicity to microorganisms

Administrative data

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1997-05-06 to 1997-12-23

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: OECD Guideline study under GLP

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1997

Report date:

1997

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test

Version / remarks:

87/302/EWG, S. 118; OECD 209

GLP compliance:

yes

Test material

Reference

Name:

Unnamed

Type:

Constituent

Details on test material:

- Name of test material (as cited in study report): PD 123408
- Physical state: White Poweder
- Composition of test material, percentage of components: not given

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

Added directly.

Test organisms

Test organisms (species):

activated sludge of a predominantly domestic sewage

Details on inoculum:

A sample of activated sewage sludge was collected from the sewage treatment plant on 1997-09-29. The sample was maintained at 22 °C with continuous aeration and fed with 50 ml/l syntetic sewage at 24 h intervals after collection. The pH was 7.2 and mean susopended solids contensts was 4.6 g/l. No adjustment of pH was considered necessary. The inoculum was diluted to approximately 3 g/l.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

3 h

Test conditions

Hardness:

120 - 180 mg/l as CaCO3

Test temperature:

24 °C

pH:

7.5 +/- 0.5

Nominal and measured concentrations:

100 mg /l (Nominal) ; 100 mg /l (Nominal)

Reference substance (positive control):

yes

Remarks:

3,5-Dichlorophenol

Results and discussion

Effect concentrations

Duration:

3 h

Dose descriptor:

IC50

Effect conc.:

> 100 mg/L

Details on results:

none

Results with reference substance (positive control):

- Results with reference substance valid? yes

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Substance is not toxic for microorganisms under the conditions used

Executive summary:

A study was performed to assess the inhibitory effect of PD 132408 on the respiration of activated sewage sludge. The study was conducted according to OECD 209 at a limit cioncentration of 100 mg/l in two dublicates. The test was run with activated sewage sludge incubated with synthetic seage under vigorous aeration in the presence of the test substance. 3,5 -Dichlorophenol was tested concurrently as a reference substance to demonstrate the satisfactory performance of the procedure. The EC50 (Respiration inhibition) value for PD 132408 was determined to be > 100 mg/l for the three hours contact time.