2-Isobutyryl-3,N-diphenylacrylamide

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1997-09-15 to 1998-02-06

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: OECD Guideline test under GLP

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1998

Report date:

1998

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

Version / remarks:

92/69/EWG, C.1; OECD Nr. 203

GLP compliance:

yes

Test material

Reference

Name:

Unnamed

Type:

Constituent

Details on test material:

- Name of test material (as cited in study report):
- Molecular formula (if other than submission substance):
- Molecular weight (if other than submission substance): - Name of test material (as cited in study report): PD 123408
- Physical state: White Poweder
- Composition of test material, percentage of components: not given

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
PHYSICO-CHEMICAL PROPERTIES
As Determined under Point 4

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

- Concentrations: 0.1; 0.21; 0.42; 0.97; 2.0 mg/l

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

TEST ORGANISM
- Common name: Rainbow Trout
- Source: Eye Research Centre, Suffolk, UK
- Length at study initiation (length definition, mean, range and SD): 3.8 cm; SD 0.33 cm
- Weight at study initiation (mean and range, SD): 0.7 g (SD 0.27 g)

ACCLIMATION
- Acclimation period: 13 days
- Acclimation conditions (same as test or not): same as test
- Type and amount of food: commercial fry pellets

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Hardness:

<= 174 mg CaCO3/L

Test temperature:

14 °C

pH:

7.5 - 8.0

Dissolved oxygen:

8.1-9.0 mg/l

Nominal and measured concentrations:

Measured: Concentrations: 0.1; 0.21; 0.42; 0.97; 2.0 mg/l

Details on test conditions:

TEST SYSTEM

- Type (delete if not applicable): open 20 l vessels
- Renewal rate of test solution (frequency/flow rate): static
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates):1
- No. of vessels per vehicle control (replicates):1
- Biomass loading rate: 0.25 g/l


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water:
- Total organic carbon:
- Particulate matter:
- Metals:
- Pesticides:
- Chlorine:
- Alkalinity:
- Ca/mg ratio:
- Conductivity:
- Culture medium different from test medium:
- Intervals of water quality measurement:


OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: 16/8

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : LC50, NOEC 0, 24, 48, 72 and 96 h

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

24 h

Dose descriptor:

LC50

Effect conc.:

> 2 mg/L

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

1.95 mg/L

Basis for effect:

mortality (fish)

Effect concentrations 3

Duration:

72 h

Dose descriptor:

LC50

Effect conc.:

1.26 mg/L

Basis for effect:

mortality (fish)

Effect concentrations 4

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

1.26 mg/L

Basis for effect:

mortality (fish)

Effect concentrations 5

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

2 mg/L

Basis for effect:

mortality (fish)

Effect concentrations 6

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

0.1 mg/L

Details on results:

%Concentration loss over test: max. 9% of nominal

Results with reference substance (positive control):

None used

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The substance is toxic to fish under the conditions used.

Executive summary:

To assess the acute toxicity a study according to OECD 203 (EU Guideline 92/92/EEC C.1) was performed with BIBEA. Concentration testsed ranged from 0.1 to 2.0 mg/l and a control. The control worked as expected. The LC40 (96h) was 1.26 mg/l with a 95% confidence interval of 0.87 to 1.92 mg/l, the NOEC was 0.1 mg/l.

The substance is toxic to fish under the conditions used.