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EC number: 675-808-5 | CAS number: 18611-43-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Skin irritation / corrosion
Administrative data
- Endpoint:
- skin corrosion: in vitro / ex vivo
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Study period:
- 6.5.-5.7.2021
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 021
- Report date:
- 2021
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 431 (In Vitro Skin Corrosion: Reconstructed Human Epidermis (RHE) Test Method)
- Version / remarks:
- 2019
- GLP compliance:
- yes
Test material
- Reference substance name:
- 1,1,3,3-tetrachloroprop-1-ene
- EC Number:
- 675-808-5
- Cas Number:
- 18611-43-3
- Molecular formula:
- C3H2Cl4
- IUPAC Name:
- 1,1,3,3-tetrachloroprop-1-ene
- Test material form:
- liquid
- Details on test material:
- concentration 99,87%
Constituent 1
In vitro test system
- Test system:
- human skin model
- Cell type:
- non-transformed keratinocytes
- Cell source:
- other: EpiDerm (Source: MatTek Corporation, USA) is a three-dimensional human epidermis model.
- Vehicle:
- not specified
- Details on test system:
- 3-minute exposure: After the 1-hour pre-incubation, each tissue was treated with an exposure
time of 3 minutes at room temperature.
60-minute exposure: After the 1-hour pre-incubation, the “Maintenance Medium” was replaced
with fresh medium (0.9 mL per well). Each tissue was treated with an exposure time of
60 minutes. The tissues were placed at 37 °C in an incubator with 5% CO2, in a ≥95%
humidified atmosphere for the exposure time. - Control samples:
- yes, concurrent negative control
- yes, concurrent positive control
- Amount/concentration applied:
- 50 μL of the test item was applied evenly to the epidermal surface with a pipette.
- Duration of treatment / exposure:
- 3-minute exposure and 60minute exposure
- Duration of post-treatment incubation (if applicable):
- 15 min
- Number of replicates:
- two for each test ( 3 min and 60 min§
Test system
- Type of coverage:
- not specified
- Vehicle:
- other: MTT diluent (MTT-100-DIL)
- Controls:
- yes, concurrent positive control
- yes, concurrent negative control
- Amount / concentration applied:
- 50 μL of the test item was added to 1 mL MTT working solution
- Duration of treatment / exposure:
- After the 1-hour pre-incubation, each tissue was treated with an exposure
time of 3 minutes at room temperature - Observation period:
- Pre-warmed (37 °C) MTT medium was added to each well below the skin units. The lid was
replaced and the plate was incubated at 37°C in an incubator with 5 % CO2, in a ≥95%
humidified atmosphere for 3 hours (± 15 minutes).
The MTT medium was removed from each well; the tissues were rinsed with DPBS (filling and
removing the wells three times), then dried and placed into a new plate.
2 ml extractant (isopropanol) was added per well, which covered inside and outside the tissue
insert. The plates were sealed shaken for two hours on a plate shaker at room temperature for
extraction.
After the extraction, the tissues were pierced, letting the extractant inside the insert to flow into
the well. The plates were shaken at approx. 120 rpm for 15 minutes. - Details on study design:
- Following the formazan extraction, 3 × 200 μL sample from each tube was placed into the wells
of a 96-well plate (labelled appropriately). The OD (Absorbance / Optical Density) of the
samples was read in a 96-well plate spectrophotometer (FLUOstar Optima microplate reader,
BMG LABTECH GmbH), at a wavelength of 570 ± 10 nm, using the extractant (isopropanol) as
blank (6 × 200 μL). Linearity range of measuring device was determined as up to 2.804 during
its validation.
Results and discussion
In vitro
Results
- Irritation / corrosion parameter:
- % tissue viability
- Run / experiment:
- 2 tissues 3 min, 60 min, negative and positive control
- Value:
- ca. 35
- Vehicle controls validity:
- valid
- Negative controls validity:
- valid
- Positive controls validity:
- valid
- Other effects / acceptance of results:
- Following exposure with 1133-Tetrachloropropene, the mean cell viability was 98.5% after the
3-minute exposure, and 34.9% after the 60-minute exposure, compared to the concurrent
negative control. This is above the thresholds of 50% (3-min) and more than 15% (60-min). As
the mean relative viability is above 50% after 3 minutes and 15% after 60 minutes of exposure,
the test item is considered not to be corrosive to skin.
Applicant's summary and conclusion
- Interpretation of results:
- GHS criteria not met
- Conclusions:
- In conclusion, in this in vitro EpiDerm™ model test with 1133-Tetrachloropropene, the
results indicate that the test item is not corrosive to the skin. - Executive summary:
In conclusion, in this in vitro EpiDerm™ model test with 1133-Tetrachloropropene, the
results indicate that the test item is not corrosive to the skin.
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