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EC number: 690-995-3 | CAS number: 756-12-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 13 April - 11 May 2012
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline study under GLP
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 012
- Report date:
- 2012
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- 1,1,1,3,4,4,4-heptafluoro-3-(trifluoromethyl)butan-2-one
- EC Number:
- 690-995-3
- Cas Number:
- 756-12-7
- Molecular formula:
- C5 F10 O
- IUPAC Name:
- 1,1,1,3,4,4,4-heptafluoro-3-(trifluoromethyl)butan-2-one
- Details on test material:
- - Name of test material (as cited in study report): MTDID 20422
- Substance type: Pure active substance
- Physical state: Volatile liquid (VP 87.5 kPa at 20 °C)
- Analytical purity: 99.99%
- Expiration date of the lot/batch: 16 Feb 2013
- Stability under test conditions: Reactive to moisture
- Storage condition of test material: Sealed container
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- sewage, predominantly domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Ssecondary effluent freshly obtained from a municipal sewage treatment plant:
'Waterschap Aa en Maas','s-Hertogenbosch, The Netherlands
- Preparation of inoculum for exposure: Secondary effluent was filtered through a coarse filter paper, the first 200 ml was discarded. The filtrate was kept aerated until inoculation.
- Inoculation level: Four mL/L, ca 2.4 mL in test bottles - Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- ca. 50 - ca. 80 mg/L
- Based on:
- test mat.
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium: Mineral medium, concentrations as per OECD 301D
- Solubilising agent (type and concentration if used): None
- Test temperature: 21.8 °C to 22.4 °C.
- pH: 7.4 - 7.5 at test initiation
- pH adjusted: No
- Aeration of dilution water: Dilution water not used, mineral medium was left at room temperature overnight before test, DO concentration measured before test began to assure saturation.
- Continuous darkness: yes
- Other: Filtered sludge aerated until inoculation, ca. 2.4 mL aerated sludge added per test bottle.
TEST SYSTEM
- Culturing apparatus: ca. 300 mL BOD bottles with glass stoppers
- Number of culture flasks/concentration: Two per concentration per day.
- Method used to create aerobic conditions: Aeration of medium before inoculation
- Measuring equipment: WTW inolab Oxi 730 supplied with a WTW CellOx 325
oxygen electrode, electrolyte type ELY/G.
- Test performed in closed vessels due to significant volatility of test substance: yes
SAMPLING
- Sampling frequency: At day 0, 7, 14, 21 and 28.
- Sampling method: Stopper removed, oxygen electrode inserted and dissolved oxygen concentration measured. pH in sample botles measured at start of test only.
CONTROL AND BLANK SYSTEM
- Inoculum blank: Yes (ten bottles)
- Abiotic sterile control: No
- Toxicity control: Yes (six bottles), same amount of test and reference substances combined as in test substance low concentration (50 mg/L) and reference substance (2 mg/L) bottles separately.
STATISTICAL METHODS:
ThOD and BOD calculated per OECD guidelines
Reference substance
- Reference substance:
- acetic acid, sodium salt
Results and discussion
% Degradation
- Parameter:
- % degradation (O2 consumption)
- Value:
- ca. 0
- Sampling time:
- 28 d
BOD5 / COD results
- Results with reference substance:
- 79% biodegradation of reference substance on day 7.
Toxicity was assessed by comparison of toxicity test bottle BOD with sum of reference substance and low test substance BOD results. No difference was observed (see Table 1), so the test substance was not considered to inhibit activated sludge.
Any other information on results incl. tables
Table 1, pH at the start and oxygen concentration during the test |
||||||||
Series |
Content |
pH at τ=0 |
Flask No. |
Oxygen concentration (mg O2/L) after x days |
||||
0 |
7 |
14 |
21 |
28 |
||||
Inoculum blank |
Mineral medium, |
7.5 |
A |
8.52 |
8.68 |
8.11 |
7.83 |
7.63 |
inoculum |
B |
8.54 |
8.66 |
8.17 |
7.79 |
7.64 |
||
mean |
8.53 |
8.67 |
8.14 |
7.81 |
7.64 |
|||
Procedure control |
Mineral medium, |
7.5 |
A |
8.57 |
7.47 |
7.00 |
||
inoculum |
B |
8.56 |
7.48 |
7.10 |
||||
sodium acetate (2 mg/l) |
mean |
8.57 |
||||||
Test substance, low |
Mineral medium, |
7.5 |
A |
8.57 |
8.69 |
8.10 |
7.94 |
7.68 |
inoculum |
B |
8.58 |
8.77 |
8.24 |
7.80 |
7.73 |
||
CAS# 756-12-7 |
mean |
8.58 |
||||||
= 10 μl/BOD bottle |
||||||||
= 15.6 mg/BOD bottle |
||||||||
= approx. 50 mg/l |
||||||||
Test substance, high |
Mineral medium, |
7.4 |
A |
8.60 |
8.68 |
8.05 |
7.92 |
7.64 |
inoculum |
B |
8.59 |
8.71 |
8.14 |
7.90 |
7.67 |
||
CAS# 756-12-7 |
mean |
8.60 |
||||||
= 15 μl/BOD bottle |
||||||||
= 23.4 mg/BOD bottle |
||||||||
= approx. 80 mg/l |
||||||||
Toxicity control |
Mineral medium, |
7.4 |
A |
8.55 |
7.41 |
6.97 |
||
inoculum |
B |
8.56 |
7.44 |
6.97 |
||||
CAS# 756-12-7 |
mean |
8.56 |
||||||
= approx. 50 mg/l plus |
||||||||
sodium acetate |
||||||||
(2 mg/l) |
Table 2, Mean values of oxygen depletion at different points in time ¹ |
||||||
Series |
Content |
Flask No. |
Oxygen depletion (mg BOD/L) after x days |
|||
7 |
14 |
21 |
28 |
|||
Procedure control |
Mineral medium, |
A |
1.24 |
1.18 |
||
inoculum |
B |
1.23 |
1.08 |
|||
sodium acetate (2 mg/l) |
||||||
Test substance, low |
Mineral medium, |
A |
0.03 |
0.09 |
-0.09 |
0.00 |
inoculum |
B |
-0.05 |
-0.05 |
0.06 |
-0.05 |
|
CAS# 756-12-7 |
||||||
= 10 μl/BOD bottle |
||||||
= 15.6 mg/BOD bottle |
||||||
= approx. 50 mg/l |
||||||
Test substance, high |
Mineral medium, |
A |
0.05 |
0.15 |
-0.04 |
0.06 |
inoculum |
B |
0.02 |
0.06 |
-0.03 |
0.03 |
|
CAS# 756-12-7 |
||||||
= 15 μl/BOD bottle |
||||||
= 23.4 mg/BOD bottle |
||||||
= approx. 80 mg/l |
||||||
Toxicity control |
Mineral medium, |
A |
1.29 |
1.20 |
||
inoculum |
B |
1.26 |
1.20 |
|||
CAS# 756-12-7 |
||||||
= approx. 50 mg/l plus |
||||||
sodium acetate |
||||||
(2 mg/l) |
1, Values corrected for oxygen depletion in blank control (mean value)
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- O2 depletion in the inoculum blank was below 1.5 mg O2/l after 28 days, residual O2 in test bottles > 0.5 mg/l at any time, control substance degraded >60% within 14 days, differences of duplicate biodegradation values less than 20% as mg O2/L
- Interpretation of results:
- under test conditions no biodegradation observed
- Conclusions:
- CAS# 756-12-7 is not readily biodegradable
- Executive summary:
Biodegradation of CAS# 756-12-7 was assessed in a closed bottle test (OECD TG301D) conducted under GLP criterion. Sodium acetate was used as reference. CAS# 756-12-7 did not measurably degrade during the test period. In toxicity controls, CAS# 756-12-7 at a concentration of 50 mg/L did not measurably prevent the reference substance from being degraded. CAS# 756-12-7 is not readily biodegradable.
The study was conducted in accord with internationally accepted guidelines under GLP criteria. It is reliable without restriction and is suitable for use in Risk Assessment, Classification & Labelling, and PBT Analysis
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