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EC number: 689-188-9 | CAS number: 149343-84-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 27 May - 24 June 1993.
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)
- Version / remarks:
- OECD Guidelines for Testing of Chemicals (1981) No. 301D "Ready Biodegradability ; Closed Bottle Test"
- Deviations:
- not specified
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- EU Method C.4-E (Determination of the "Ready" Biodegradability - Closed Bottle Test)
- Version / remarks:
- Method C.6 of Commission Directive 84/ 449/ EEC (which constitutes Annex V of Council Directive 67/ 548/ EEC) .
- Deviations:
- not specified
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- No further details specified in the study report.
- Oxygen conditions:
- anaerobic
- Inoculum or test system:
- activated sludge, domestic (adaptation not specified)
- Details on inoculum:
- Type: Activated sludge bacteria.
Source: The aeration stage of the Severn Trent Water Plc sewage treatment plant at Belper, Derbyshire, treating predominantly domestic sewage.
Date of collection: 27 May 1993.
Preparation: The sample was homogenized for 5 minutes and the suspended solids allowed to settle for approximately 30 minutes. The supernatant was then centrifuged to remove any fine solids prior to use. - Duration of test (contact time):
- 28 d
- Initial conc.:
- 3 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- Vessels: 250-300 ml BOD bottles (darkened glass) with ground glass stoppers.
Test concentrations:
(a) Non - inoculated culture medium .
(b) Inoculated culture medium.
(c) 3 mg/l test material
(d) 3 mg/l sodium benzoate.
Test media b, c and d were inoculated with activated sludge filtrate at the rate of 1 drop of inoculum per litre.
Procedure: BOD bottles were filled by siphon and firmly stoppered to exclude all air bubbles. Sufficient bottles were prepared to allow a single oxygen determination per bottle to be made at 0, 5, 15 and 28 days for each test medium (duplicate bottles at each sampling time).
Bottles were incubated in a water bath at 21 °C for 0, 5, 15 or 28 days as appropriate.
Observations: Dissolved oxygen concentrations for each test medium were determined, in duplicate, at 0, 5, 15 and 28 days by means of a Yellow Springs BOD Probe (Model 54). - Reference substance:
- benzoic acid, sodium salt
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 0
- Sampling time:
- 28 d
- Details on results:
- JPR Blue 100 attained 0% degradation after 28 days and so, therefore, cannot be considered as ready biodegradable under the strict terms and conditions of OECD Guideline No . 3010.
- Results with reference substance:
- Sodium benzoate attained 84% degradation after 28 days thereby confirming the suitability of the test method and culture conditions.
- Validity criteria fulfilled:
- not specified
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- RESULTS
TEST CONCENTRATION: 3 mg/l
OXYGEN DEPLETION (28 Days): 0.00 mg O2/l
THEORETICAL OXYGEN DEMAND (ThOD): 4.68 mg O2/l
PERCENTAGE BIODEGRADATION (28 Days): 0% - Executive summary:
TEST TYPE: Ready biodegradation; Closed bottle Test
TEST MATERIAL: JPR Blue 100
METHOD: Closed Bottle Test according to OECD Guideline No 301D referenced as Method C.6 of Commission Directive 84/ 449/ EEC (which constitutes Annex V of Council Directive 67/ 548/ EEC)
DURATION: 28 days.
TEMPERATURE: 21 °C.
RESULTS
TEST CONCENTRATION: 3 mg/l
OXYGEN DEPLETION (28 Days): 0.00 mg O2/l
THEORETICAL OXYGEN DEMAND (ThOD): 4.68 mg O2/l
PERCENTAGE BIODEGRADATION (28 Days): 0%
Reference
Dissolved Oxygen Measurements (mg O2/l)
Test Series |
DAY |
||||
0 |
5 |
15 |
28 |
||
(a) Culture medium without inoculum |
R1 R1 Mean |
8.90 8.90 8.900 |
8.90 8.90 8.900 |
8.70 8.80 8.750 |
8.70 8.65 8.675 |
(b) Culture medium with inoculum |
R1 R2 Mean |
8.90 8.90 8.900 |
8.85 8.90 8.875 |
8.60 8.60 8.600 |
8.50 8.55 8.525 |
(c) Test material, JPR Blue 100 (3 mg/l) with inoculum |
R1 R2 Mean |
8.90 8.90 8.900 |
8.90 8.80 8.850 |
8.70 8.70 8.700 |
8.55 8.60 8.575 |
(d) Standard material, Sodium benzoate (3 mg/l) with inoculum |
R1 R2 Mean |
8.90 8.90 8.900 |
5.45 5.35 5.400 |
4.90 5.10 5.000 |
4.20 4.40 4.300 |
R1and R2= Replicates 1 and 2
Mean Oxygen Depletion and Percentage Biodegradation Values
Test Series |
DAY |
|||
5 |
15 |
28 |
||
(a) Culture medium without inoculum |
} O2depletion (mg O2/l) } % degradation |
0.000 - |
0.150 - |
0.225 - |
(b) Culture medium with inoculum |
} O2depletion (mg O2/l) } % degradation |
0.025 - |
0.300 - |
0.375 - |
(c) Test material, JPR Blue 100 (3 mg/l) with inoculum |
} O2depletion (mg O2/l) } % degradation |
0.025 1 |
0.000 0 |
0.000 0 |
(d) Standard material, Sodium benzoate (3 mg/l) with inoculum |
} O2depletion (mg O2/l) } % degradation |
3.475 69 |
3.600 72 |
4.225 84 |
i) Test and Standard materials
Oxygen depletion = (a – c or d) – (a – b)
ii) Inoculated and non-inoculated culture medium
Oxygen depletion = initial O2concentration – measured value
iii) % Degradation = Oxygen depletion
---------------------------------------------- x 100
Conc. of material x ThOD NO3
Description of key information
OXYGEN DEPLETION (28 Days): 0.00 mg O2/l
THEORETICAL OXYGEN DEMAND (ThOD): 4.68 mg O2/l
PERCENTAGE BIODEGRADATION (28 Days): 0%
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
Additional information
TEST TYPE: Ready biodegradation; Closed bottle Test
TEST MATERIAL: JPR Blue 100
METHOD: Closed Bottle Test according to OECD Guideline No 301D referenced as Method C.6 of Commission Directive 84/ 449/ EEC (which constitutes Annex V of Council Directive 67/ 548/ EEC)
DURATION: 28 days.
TEMPERATURE: 21 °C.
RESULTS
TEST CONCENTRATION: 3 mg/l
OXYGEN DEPLETION (28 Days): 0.00 mg O2/l
THEORETICAL OXYGEN DEMAND (ThOD): 4.68 mg O2/l
PERCENTAGE BIODEGRADATION (28 Days): 0%
[Type of water: freshwater]
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