Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 248-374-6 | CAS number: 27253-32-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to reproduction: other studies
Administrative data
- Endpoint:
- toxicity to reproduction: other studies
- Type of information:
- experimental study
- Adequacy of study:
- disregarded due to major methodological deficiencies
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Not to GLP, No a standard study but follows basic scientific principles
Data source
Reference
- Reference Type:
- publication
- Title:
- Effect of zinc on manganese induced testicular injury in rats
- Author:
- Chandra SV , Saxena DK and Hasan MZ
- Year:
- 1 975
- Bibliographic source:
- Ind. Health, 13: 51-56
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- 4 groups of 10 rats were used, group 1 was exposed to the control, group 2 was exposed to MnSO4, group 3 was exposed to ZnSO4 and group 4 was exposed to both MnSO4 and ZnSO4 daily via i.p injection for 30 days. All animals were sacrificed at the end of the exposure period; 1 testis was kept for the estimation of the manganese of zinc content and the other testis was fixed for histology.
- GLP compliance:
- no
- Type of method:
- in vivo
Test material
- Reference substance name:
- manganese sulphate tetrahydrate
- IUPAC Name:
- manganese sulphate tetrahydrate
- Details on test material:
- - Name of test material (as cited in study report): Manganese sulphate (B.D.H.)
- Molecular formula (if other than submission substance): MnSO4.4H2O
- Analytical purity: Analytical reagent quality
Constituent 1
Test animals
- Species:
- rat
- Strain:
- other: Hooded
- Sex:
- male
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Industrial Toxicology Research Centre bred
- Age at study initiation: Not reported
- Weight at study initiation: 150 ± 20 g
- Diet : Pellet diet from Hindustan Levers India Ltd. ad libitum
- Water : Tap water ad libitum
Administration / exposure
- Route of administration:
- intraperitoneal
- Vehicle:
- physiological saline
- Details on exposure:
- PREPARATION OF DOSING SOLUTIONS: 6 mg/kg of MnSO4.4H2O dissolved in 0.2 mL of physiological saline. 2 mg/kg of ZnSO4.7H2O dissolved in 0.2 mL physiological saline.
- Dosing scheme: Group 1 was exposed to the control, group 2 was exposed to MnSO4, group 3 was exposed to ZnSO4 and group 4 was exposed to both MnSO4 and ZnSO4 daily via i.p injection for 30 days. - Analytical verification of doses or concentrations:
- no
- Details on analytical verification of doses or concentrations:
- Not applicable
- Duration of treatment / exposure:
- 30 days
- Frequency of treatment:
- Single daily treatment for 30 days for groups I, II and III. For group IV, animals were dosed once in the morning with ZnSO4 (same concentration and volume) and dosed once in the afternoon of the same day with MnSO4 (same concentration and volume) for 30 days.
- Duration of test:
- Not applicable
Doses / concentrationsopen allclose all
- Remarks:
- Doses / Concentrations:
6 mg/kg
Basis:
nominal conc.
MnSO4 in 0.2 mL physiol. saline
- Remarks:
- Doses / Concentrations:
2 mg/kg
Basis:
nominal conc.
ZnSO4 in 0.2 mL physiol. saline
- No. of animals per sex per dose:
- 10 animals were dosed in each group
- Control animals:
- yes, concurrent vehicle
- Details on study design:
- At the end of the experimental period, all animals were sacrificed under ether anaesthesia. One testis was kept for the estimation of manganese and zinc contents and the other testis from each rat was fixed in 10% neutral formalin for histology. After routine processing the tissues were embedded in paraffin and sections were cut at 5 µ. The staining was done with haematoxylin and eosin and for the histochemical demonstration of calcium by the method of Von Kossa.
- Statistics:
- Data were analysed by Fisher's test. The statistical significance was calculated between two values of control and treated rats and a p value of 0.05 or less was considered significant.
Results and discussion
Effect levels
- Dose descriptor:
- conc. level:
- Effect level:
- 6 mg/kg bw/day (nominal)
- Sex:
- male
- Basis for effect level:
- other: A decrease in the number of spermatocytes was noted along with degeneration in the seminiferous epithelium
Observed effects
Any other information on results incl. tables
Table 1: Manganese and zinc contents in testis
Groups (No. of Rats) |
Treatment given daily for 30 days |
Manganese contents µg/g of wet tissue |
Zinc contents µg/g of wet tissue |
I (10) |
0.2 mL physiological saline intraperitoneally (controls) |
0.302 ± 0.06 |
6.753 ± 0.72 |
II (10) |
MnSO4.4H2O, 6 mg Mn/kg intraperitoneally |
0.723 ± 0.01 p < 0.01 |
8.111 ± 0.6 p < 0.05 |
III (10) |
ZnSO4.7H2O, 2 mg Zn/kg intraperitoneally |
0.429 ± 0.01 N.S. |
9.80 ± 0.5 p < 0.01 |
IV (10) |
MnSO4.4H2O, 6 mg Mn/kg and ZnSO4.7H2O, 2 mg Zn/kg intraperitoneally |
0.573 ± 0.01 p < 0.01 |
11.09 ± 0.32 p < 0.001 |
Values represent the mean ± S.E. of 6 rats p values of 0.05 or less are significant |
Applicant's summary and conclusion
- Conclusions:
- The increase in manganese content in testis of rats treated with both manganese and zinc sulphates is less than in testicular tissue of animals administered with manganese sulphate alone. It was also observed that the increase in the testicular contents of zinc is more in animals treated with both the salts as compared to the zinc contents of testis after treatment with zinc sulphate alone. The simultaneous administration of zinc and manganese in experimental animals results in less accumulation of manganese and more concentration of zinc in testis. Excess of zinc may be preventing the interference of manganese with the energy synthesizing enzyme system of the cells of seminiferous tubules and therefore preventing cellular death.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.