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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Neurotoxicity

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Administrative data

Endpoint:
neurotoxicity: short-term inhalation
Type of information:
migrated information: read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Conducted to a reasonable scientific standard

Data source

Reference
Reference Type:
publication
Title:
Oxidative Stress Is Induced in the Rat Brain Following Repeated Inhalation Exposure to Manganese Sulfate
Author:
Dobson A, Weber S, Dorman D, Lash L, Erikson K & Aschner M
Year:
2003
Bibliographic source:
Biological Trace Element Research, 93: 113-125.

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
Eight week old male rats inhaled manganese (Mn) in the form of MnSO4 at 0.0, 0.03, 0.3 or 3.0 mg Mn/m3 for 6 hours per day for 14 consecutive days.
GLP compliance:
not specified
Limit test:
no

Test material

Constituent 1
Reference substance name:
manganese sulphate
IUPAC Name:
manganese sulphate
Constituent 2
Reference substance name:
Manganese sulphate
EC Number:
232-089-9
EC Name:
Manganese sulphate
IUPAC Name:
232-089-9
Constituent 3
Reference substance name:
7785-87-7
Cas Number:
7785-87-7
Molecular formula:
MnSO4
IUPAC Name:
7785-87-7

Test animals

Species:
rat
Strain:
other:
Sex:
male

Administration / exposure

Route of administration:
inhalation: dust
Vehicle:
unchanged (no vehicle)
Analytical verification of doses or concentrations:
yes
Duration of treatment / exposure:
14 consecutive days
Frequency of treatment:
6 hours per day
Doses / concentrationsopen allclose all
Remarks:
Doses / Concentrations:
0.092 mg MnSO4/m3 (0.03 mg Mn/m3)
Basis:
analytical conc.
Remarks:
Doses / Concentrations:
0.92 mg MnSO4/m3 (0.3 mg Mn/m3)
Basis:
analytical conc.
Remarks:
Doses / Concentrations:
9.2 mg MnSO4/m3 (3 mg Mn/m3)
Basis:
analytical conc.
No. of animals per sex per dose:
Not reported
Control animals:
yes, concurrent no treatment

Results and discussion

Effect levels

open allclose all
Dose descriptor:
other: Increase in glutamine synthetase protein level in olfactory bulbs
Effect level:
0.3 mg/m³ air
Based on:
other: Mn2+ content
Sex:
male
Basis for effect level:
other: overall effects neurochemistry;
Remarks on result:
other:
Dose descriptor:
other: Increase in glutamine synthetase protein level in hypothalamus
Effect level:
0.03 mg/m³ air (analytical)
Based on:
other: Mn2+ content
Sex:
male
Basis for effect level:
other: overall effects neurochemistry
Remarks on result:
other:
Dose descriptor:
other: Decrease in glutamine synthetase protein level in cerebellum
Effect level:
3 mg/m³ air (analytical)
Based on:
other: Mn2+ content
Sex:
male
Basis for effect level:
other: overall effects neurochemistry
Remarks on result:
other:
Dose descriptor:
other: Decrease in total glutathione levels in the hypothalamus
Effect level:
3 mg/m³ air (analytical)
Based on:
other: Mn2+ content
Sex:
male
Basis for effect level:
other: overall effects neurochemistry
Remarks on result:
other:

Any other information on results incl. tables

Areas where significant increases in glutamine synthetase protein levels were observed:

Olfactory bulbs at 0.3 mg Mn /m3 air

Hypothalamus at 0.03 mg Mn/m3 air

Significant decrease in glutamine syhthetase protein observed in the cerebellum at 3 mg Mn/m3..

Significant decrease in total glutathione levels observed in the hypothalamus at 3 mg Mn/m3.

Applicant's summary and conclusion

Conclusions:
It cannot be unequivocally stated that the data supports the ability of MnSO4 to induce oxidative stress in the hypothalamus. The relevance of the findings to primates has yet to be determined. The lack of changes in any of the measured changes in the striatum indicates that MnSO4 inhalation, even at very high doses, does not induce oxidative stress in this brain region.