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EC number: 947-953-7 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2018-03-19 to 2018-03-26
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Referenceopen allclose all
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 018
- Report date:
- 2018
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 018
- Report date:
- 2018
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- 2004
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Version / remarks:
- 2008
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Reaction mass of Reaction product of 1-chloro-3-{[1-chloro-3-(dodecyloxy)propan-2-yl]oxy}propan-2-ol with methyl diethanolamine and [3-(dodecyloxy)-2-hydroxypropyl]bis(2-hydroxyethyl)methylammonium chloride
- IUPAC Name:
- Reaction mass of Reaction product of 1-chloro-3-{[1-chloro-3-(dodecyloxy)propan-2-yl]oxy}propan-2-ol with methyl diethanolamine and [3-(dodecyloxy)-2-hydroxypropyl]bis(2-hydroxyethyl)methylammonium chloride
- Test material form:
- other: solid, very viscous yellow paste
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: Experiment 1: 1.0 / 2.2 / 4.6 / 10 / 22 mg/L; Experiment 2: 0.1 / 0.22 / 0.46 / 1.0 /2.2 mg/L
- Sampling method: taken at the beginning and at the end of the test
- Sample storage conditions before analysis: storage in a fridge for samples not measured immediately. The mean stability of the test item in Daphnia medium was determined to be 119.4 % after 48h.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: Stock solutions containing 22.3 mg/L and 22.5 mg/L test item in dilution water were pre-pared for experiment 1 and experiment 2, respectively. The lower concentrations were prepared by dilution of this stock solution with dilution water.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna
- Strain/clone: Berlin
- Source: Umweltbundesamt Berlin
- Food type: unicellular green algae (Desmodesmus subspicatus)
SELECTION OF DAPHNIA
- Experiment 1: 18 hours 40 minutes before the start of the test, the adult animals were separated from the young. 0.75 hours before test start, the adults were caught with the help of a glass tube, and the newborn daphnia, aged between 0 and 17 hours 55 minutes, were sieved from the medium and immediately placed into a beaker containing dilution water. After the settling-in period, animals which showed no apparent damage were used for the test.
- Experiment 2: 18 hours before the start of the test, the adult animals were separated from the young. 1 hour before test start, the adults were caught with the help of a glass tube, and the newborn daphnia, aged between 0 and 17 hours, were sieved from the medium and immediately placed into a beaker containing dilution water. After the settling-in period, animals which showed no apparent damage were used for the test.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- 250 mg CaCO3/L
- Test temperature:
- Experiment 1: 20.3 - 21.8°C
Experiment 2: 19.7 - 20.3°C - pH:
- Experiment 1: 7.8-8.0
Experiment 2: 7.8-7.9 - Dissolved oxygen:
- Experiment 1: 8.5-9.2 mg/L
Experiment 2: 8.8-9.1 mg/L - Nominal and measured concentrations:
- Experiment 1: 1.0 / 2.2 / 4.6 / 10 / 22 mg/L (nominal); 1.2, 2.7, 5.4, 13.1, 29.2 mg/L (measured)
Experiment 2: 0.1 / 0.22 / 0.46 / 1.0 /2.2 mg/L (nominal); 0.1, 0.2, 0.5, 1.1, 2.5 mg/L (measured) - Details on test conditions:
- TEST SYSTEM
- Test vessel: glass beakers, nominal volume 50 mL, tall shape
- Fill volume: 20 ± 5 mL test solution/ dilution water
- Renewal rate of test solution (frequency/flow rate): none
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- pH: 7.8
- Dissolved oxygen concentration: 9.2 mg/L
- Total hardness: 250 mg/L as CaCO3
- Culture medium different from test medium: yes, M4-medium for husbandry
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16/8 hours
EFFECT PARAMETERS MEASURED
- Biological Assessment: after 24 h and 48 h: count the immobilised daphnids
RANGE-FINDING STUDY
- Test concentrations: 1 / 10 / 100 mg/L.
- Results used to determine the conditions for the definitive study: At 1 mg/L 0% toxicity was observed and at 10 and 100 mg/L 100% toxicity was observed. - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.795 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.5 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 1.1 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- IMMOBILITY
- Experiment 1: All concentrations showed toxicity between 95 and 100 % immobilisation. Trapping on the surface or clotting was not observed. None of the animals was immobilised in the blank control.
- Experiment 2: The two highest concentrations showed toxicity between 90 and 100 % immobilisation. None of the animals was immobilised in the lower concentrations and in the blank control.
ANALYTICAL DETERMINATIONS
- Experiment 1: 123 - 135 % of the nominal concentrations at the beginning of the test and 108 - 131 % of the nominal concentration after 48 hours.
- Experiment 2: 79 - 110 % of the nominal concentrations at the beginning of the test and 76 - 115 % of the nominal concentration after 48 hours. - Results with reference substance (positive control):
- - Results with reference substance valid?
yes
- Relevant effect levels: 24h-EC50: 1.6 mg/L - Reported statistics and error estimates:
- Since immobility in experiment 1 was observed from 95 to 100% in all treatments, no statistical evaluation was conducted. However biological results were given in a range of concentrations or determined as lower orlower as or higher than a certain concentration. The estimation of the biological results from experiment 2 was accomplished using the software ToxRat® Professional, version 3.2.1.
Any other information on results incl. tables
Biological results
Immobility experiment 1:
Nominal Concentration in mg/L |
Immobility 24 hours |
Immobility 48 hours |
||||||||
absolute |
in % |
absolute |
in % |
|||||||
Blank control |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
1.0 |
0 |
0 |
0 |
0 |
0 |
5 |
5 |
5 |
4 |
95 |
2.2 |
5 |
5 |
5 |
5 |
100 |
5 |
5 |
5 |
5 |
100 |
4.6 |
5 |
5 |
5 |
5 |
100 |
5 |
5 |
5 |
5 |
100 |
10 |
5 |
5 |
5 |
5 |
100 |
5 |
5 |
5 |
5 |
100 |
22 |
5 |
5 |
5 |
5 |
100 |
5 |
5 |
5 |
5 |
100 |
Immobility experiment 2:
Nominal Concentration in mg/L |
Immobility 24 hours |
Immobility 48 hours |
||||||||
absolute |
in % |
absolute |
in % |
|||||||
Blank control |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0.1 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0.22 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0.46 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
1.0 |
0 |
0 |
2 |
0 |
10 |
4 |
4 |
5 |
5 |
90 |
2.2 |
5 |
5 |
5 |
5 |
100 |
5 |
5 |
5 |
5 |
100 |
Analytical results:
Measured concentrations experiment 1:
Nominal |
Measured concentrations |
Measured concentrations |
% of Nominal concentration |
% of Nominal concentration |
mg/L |
mg/L |
mg/L |
% |
% |
Blank control |
0.00 |
0.00 |
-- |
-- |
1 |
1.23 |
1.23 |
123 |
123 |
2.2 |
2.75 |
2.72 |
125 |
123 |
4.6 |
5.86 |
4.99 |
127 |
108 |
10 |
13.25 |
12.91 |
132 |
129 |
22 |
29.71 |
28.72 |
135 |
131 |
Measured concentrations experiment 2:
Nominal |
Measured concentrations |
Measured concentrations |
% of Nominal concentration |
% of Nominal concentration |
mg/L |
mg/L |
mg/L |
% |
% |
Blank control |
0.00 |
0.00 |
-- |
-- |
0.1 |
0.08 |
0.08 |
79 |
76 |
0.22 |
0.22 |
0.19 |
101 |
86 |
0.46 |
0.46 |
0.44 |
101 |
95 |
1.0 |
1.06 |
1.08 |
106 |
108 |
2.2 |
2.42 |
2.52 |
110 |
115 |
Effect values:
Biological Results Test Item Experiment 1 (based on geometric mean of measured concentrations):
Parameter |
Value |
24h EC50 |
1.2 - 2.7 mg/L |
48h EC50 |
< 1.2 mg/L |
48h NOEC |
< 1.2 mg/L |
48h LOEC |
≤ 1.2 mg/L |
Biological results test item experiment 2 (based on geometric mean of measured concentrations):
Parameter |
Value |
95% confidence interval |
24h EC50 |
1.278 mg/L |
n.d. |
48h EC50 |
0.795 mg/L |
0.689 - 0.922 mg/L |
48h NOEC |
0.5 mg/L |
-- |
48h LOEC |
1.1 mg/L |
-- |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The EC50 (48 h) for immobilisation was determined to be 0.795 mg/L. The corresponding NOEC (48 h) was 0.5 mg/L.
- Executive summary:
The short term toxicity of test item to Daphnia magna STRAUS was assessed according to OECD 202 resp. EU C.2 over a period of 48 hours.
Two valid experiments were performed. In the first experiment all concentrations showed toxicity between 95 and 100 % immobilisation. In the second experiment the two highest concentrations showed toxicity between 90 and 100 % immobilisation.
The content of the test item in the test solutions was analytically verified using LC-MS-MS-determination. The determination of the biological results was based on the geometric mean of the measured concentrations.
The EC50 (48 h) for immobilisation was determined to be 0.795 mg/L. The corresponding NOEC (48 h) was 0.5 mg/L.
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