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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
28 Feb - 02 Mar 1995
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1995
Report Date:
1995

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Qualifier:
according to
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
GLP compliance:
yes (incl. certificate)

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
solid: particulate/powder
Details on test material:
Basic Blue 123 Cl

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
- Concentrations: all
- Sampling method: taken from approximate centre of test vessel
- Sample storage conditions before analysis: kept at -18°C to -25°C until analysis

Test solutions

Vehicle:
yes
Remarks:
Tween 80
Details on test solutions:
0.5 g test substance were mixed with 200 mL test medium in a beaker
mixture was homogenized for 8 minutes by ultrasonic treatment and filled up to 500 mL with test medium

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: water flea
- Source: own breed
- Feeding during test: no
- Food type: suspension of green algae and Tetramin extract
- Amount: amount needed for 24 h
- Frequency: daily

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h

Test conditions

Hardness:
231 mg/L CaCO3 (test medium)
Test temperature:
21°C
pH:
7.8 - 8.0
Dissolved oxygen:
97 - 99% saturation
Nominal and measured concentrations:
Nominal: 0 (control), 0 (solvent control), 4.3, 9.4, 21, 45 and 100 mg/L
Measured (arithmetic mean):
Details on test conditions:
TEST SYSTEM
- Test vessel: glass beaker
- Material, size, headspace, fill volume: 250 mL, fill volume: 60-100 mL
- Aeration: no
- No. of organisms per vessel: 1ß
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Aqua bidist.
- Alkalinity: 0.8 mmol/L
- Ca/mg ratio: 4:1
- Culture medium different from test medium: no
- Intervals of water quality measurement: beginning of each test

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 12/12 h

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : immobility (24, 48h)


RANGE-FINDING STUDY
- Test concentrations: 0.01 - 100 mg/L
- Results used to determine the conditions for the definitive study: yes
Reference substance (positive control):
yes
Remarks:
potassium dichromate

Results and discussion

Effect concentrationsopen allclose all
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
8.3 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
4.3 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
- Behavioural abnormalities: no
- Mortality of control / solvent control: no
Results with reference substance (positive control):
24-h EC50: 0.95 mg/L corresponding to a normal sensitivity (25 Jan 1995)
Reported statistics and error estimates:
Probit model

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The 48-h EC50 to Daphnia magna was determined at 8.3 mg/L based on nominal concentrations (analytically verified). The substance is acutely harmful to aquatic invertebrates.