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EC number: 226-430-0 | CAS number: 5398-29-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 0, 1,56 and 100 mg/L treatment
- Sampling method: samples were taken at the beginning and at the end of the test period.
- Sample preparation: Standards were dissolved and diluted in the carrier after addition of a solution of internal standard the samples were injected directly.
- Storage condition of samples: Refrigerator - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: A stock solution was prepared by adding 100 mg/L of test substance to water and stirring for 20 min at 20 °C. The test concentrations were prepared by subsequent dilution of the stock solution. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: water flea
- Source: The clone of Daphnia magna STRAUS 1820 used was supplied by the Institut National de Recherche Chimique Appliquée, France, in 1978. From this date on this clone was cultured and bred continuously in the Laboratory of Ecotoxicology of the Experimental Toxicology and Ecology of BASF SE in Ludwigshafen.
- Age at study initiation (mean and range, SD): < 24 hours (at least 3rd brood progeny)
- Feeding during test: none - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- 2.20 - 3.20 mmol/L
- Test temperature:
- 20 ± 2 °C
- pH:
- 7.9 - 8.5
- Dissolved oxygen:
- 9.2 - 15.3 mg/L
- Nominal and measured concentrations:
- Nominal: 1.56, 3.13, 6.25, 12.5, 25, 50 and 100 mg/L
Measured corresponding to following nominal concentrations: 0 (control), 1.56 and 100 mg/L: < 1, 2 and 92 mg/L (mean value) - Details on test conditions:
- TEST SYSTEM
- Test vessel: 20 mL test tubes with 10 mL test solution
- Aeration: none
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: synthetic medium according to guideline
- Ca/mg ratio: 4:1
- Conductivity: 550 - 650 µS/cm
- Culture medium different from test medium: no
- Intervals of water quality measurement: temperature was recorded after 0, 24 and 48h test duration. Oxygen and pH were measured at the beginning and at the end of the test.
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16:8 light:dark
- Light intensity: 5-6 µE/m2*s (400-700 nm)
EFFECT PARAMETERS MEASURED: Immobilisation was recorded after 3, 6, 24 and 48h exposure time. - Reference substance (positive control):
- no
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 20.8 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC0
- Effect conc.:
- 6.25 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC100
- Effect conc.:
- 50 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Reported statistics and error estimates:
- For the statistical evaluation of the data and calculation of the EC50 the Moving Average method was used.
- Validity criteria fulfilled:
- yes
- Conclusions:
- The test substance showed to be acutely harmful to aquatic invertebrates (48h-EC50 = 20.8 mg/L) in a GLP guideline study using Daphnia magna as test organism.
Reference
Since the analytically determined concentrations of the test substance in the test solutions were within +20% of the nominal concentrations, the effect concentration can be expressed relative to the nominal concentration.
Description of key information
EC50 (48h) = 20.8 mg/L (nominal, analytically verified) for Daphnia magna (EU method C.2)
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 20.8 mg/L
Additional information
One study investigating the short-term toxicity of 3-(amidinothio)propionic acid (CAS 5398-29-8) to aquatic invertebrates is available. The study was performed according to GLP and EU method C.2 using Daphnia magna as test organism (BASF 1995). Test concentrations between 1.56 and 100 mg/L were tested. Nominal concentrations were verified analytically. The 48h-EC50 value is determined to be 20.8 mg/L based on nominal concentrations.
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