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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
adsorption / desorption: screening
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1995
Report date:
1995

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 121 (Estimation of the Adsorption Coefficient (Koc) on Soil and on Sewage Sludge using High Performance Liquid Chromatography (HPLC))
GLP compliance:
yes
Type of method:
HPLC estimation method
Media:
soil

Test material

Constituent 1
Chemical structure
Reference substance name:
3-(amidinothio)propionic acid
EC Number:
226-430-0
EC Name:
3-(amidinothio)propionic acid
Cas Number:
5398-29-8
Molecular formula:
C4H8N2O2S
IUPAC Name:
3-(carbamimidoylsulfanyl)propanoic acid
Test material form:
solid: particulate/powder
Remarks:
migrated information: powder
Details on test material:
- Name of test material (as cited in study report): Carboxyethylisothiuroniumbetain
- Physical state: powder
- Analytical purity: > 98%
- Impurities (identity and concentrations): < 1% thiocarbamide
- Lot/batch No.: 88-3054
Radiolabelling:
no

Study design

Test temperature:
not specified

HPLC method

Details on study design: HPLC method:
EQUIPMENT
- Apparatus: HP 1090 (liquid chromatopraph)
- Type, material and dimension of analytical (guard) column: 125 mm x 4 mm cyanopropyl, 5 µm diameter (LiChrospher 100 CN, Merck)
- Detection system: UV

MOBILE PHASES
- Type: stationary phase
- Experiments with additives carried out on separate columns: no
- pH: Citrate buffer, pH = 7.0
- Eluent: 60 vol .-%: 10 mmol/L. 40 vol .-%: acetonitrile
- Flow rate: 1 mL/min
- Injection volume: 25 µL
- Calculation of retention times: 0.814 – 0.914 min

DETERMINATION OF DEAD TIME
- Method: by inert substances which are not retained by the column (nitrate)

REFERENCE SUBSTANCES
- Identity: Monolinuron, Atrazin, Isoproturon, Monuron, Carbendazim, Tradimenol, Fenamiphos, Triadimefon, Linuron, Fensulfothion, Mercaptodimetur, Fuberidazol, Disulfoton, Azinphos-methyl, Isofenphos, Fenthion, Pyrazophos, Anthrachinon, Trifluralin, alpha-Endosulfan, Quintozen, Sulprofos

DETERMINATION OF RETENTION TIMES
- Quantity of test substance introduced in the column: 22.5 mg in 1000 mL
- Quantity of reference substances: In each case, approximately 5 to 25 mg were weighed, transferred to a 10 mL volumetric flask and make up to the mark with water.

REPETITIONS
- Number of determinations: 2

EVALUATION
- Calculation of capacity factors k': k' = (tr-to)/to [tr =n retention time of test substance, to = retention time of nitrate]
- Determination of the log Koc value: log Koc = logK'/(slope+axis intercept)

Results and discussion

Adsorption coefficient
Type:
log Koc
Value:
-0.4

Results: HPLC method

Details on results (HPLC method):
- Retention times of reference substances used for calibration: see attachment
- Details of fitted regression line (log k' vs. log Koc): y = 0.2381x-0.1805, R2 = 0.5352
- Average retention data for test substance: 1.396 min

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The log Koc value is determined to be - 0.4.