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EC number: 214-093-2 | CAS number: 1079-66-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2018
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 018
- Report date:
- 2018
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)
- GLP compliance:
- yes
Test material
- Reference substance name:
- Chlorodiphenylphosphine
- EC Number:
- 214-093-2
- EC Name:
- Chlorodiphenylphosphine
- Cas Number:
- 1079-66-9
- Molecular formula:
- C12H10ClP
- IUPAC Name:
- diphenylphosphinous chloride
- Test material form:
- liquid
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- At the start and the end of the test.
Samples will be taken from all concentrations and the control
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: A loading rate of 100 mg CHLORODIPHENYLPHOSPHINE per litre of test medium will be prepared and magnetically stirred for one day. The water saturated solution (SS) will be taken out by siphoning and used as the highest test concentration. Lower test concentrations will be prepared by proportional dilution of the SS in test medium.
The tests will be carried out without adjustment of the pH, except if pH values of test solutions are outside the optimal pH range for the species to be tested. No correction will be made for the purity/composition of the test item.
- Controls: Yes
Test organisms
- Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
- Common name: Pseudokirchneriella subcapitata
- Source (laboratory, culture collection): In-house laboratory culture.
- Age of inoculum (at test initiation):
- Method of cultivation: Algae stock cultures are started by inoculating growth medium with algal cells from a pure culture on agar. The suspensions are continuously aerated and exposed to light in a climate room at a temperature of 21-24°C.
- Light intensity 60 to 120 μE/m2/s when measured in the photosynthetically effective wavelength range of 400 to 700 nm.
- Stock culture medium M1; according to the NPR 6505, formulated using Milli-RO water
- Pre-culture 2 to 4 days before the start of the test, cells from the algal stock culture are inoculated in culture medium at a cell density of 1 x 104 cells/mL. The pre-culture is maintained under the same conditions as used in the test. The cell density is measured immediately before use.
- Pre-culture medium M2; according to the OECD 201 Guideline, formulated using Milli-RO
Study design
- Test type:
- static
- Water media type:
- freshwater
- Total exposure duration:
- 72 h
Test conditions
- Hardness:
- 24 mg CaCO3/L
- Test temperature:
- 21-24 °C, constant within 2°C
- pH:
- Between 6.0 and 9.0
- Salinity:
- Not applicable, freshwater
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 100 mL, all-glass (standard)
- Initial cells density: An initial cell density of 1 x 104 cells/mL using an exponentially growing pre-culture.
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6
GROWTH MEDIUM
- Standard medium used: yes
OTHER TEST CONDITIONS
- pH: Between 6.0 and 9.0
- Photoperiod: Continuously using TLD-lamps with a light intensity within the range of 60 to 120 μE.m-2.s-1
EFFECT PARAMETERS MEASURED :
- Determination of cell concentrations: [counting chamber]
TEST CONCENTRATIONS
- Spacing factor for test concentrations:
- Justification for using less concentrations than requested by guideline:
- Range finding study
- Test concentrations:
- Results used to determine the conditions for the definitive study: - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 53 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 17 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- Under the conditions of the present study with Pseudokirchneriella subcapitata, the degradation product of CHLORODIPHENYLPHOSPHINE inhibited both growth rate and yield of this fresh water algae species at 6.5 mg/L and higher.
- Executive summary:
Under the conditions of the present study with Pseudokirchneriella subcapitata, the degradation product of CHLORODIPHENYLPHOSPHINE inhibited both growth rate and yield of this fresh water algae species at 6.5 mg/L and higher.
· The 72h-NOEC for growth rate inhibition was 2.2 mg/L when based on statistical analysis and 6.5 mg/L when based on biological relevance.
· The 72h-EC10 for growth rate inhibition (ERC10) was 17 mg/L with a 95% confidence interval ranging from 16 to 18 mg/L.
· The 72h-EC50 for growth rate inhibition (ERC50) was 53 mg/L with a 95% confidence interval ranging from 52 to 55 mg/L.
· The 72h-NOEC for yield inhibition was 2.2 mg/L when based on both statistical analysis and biological relevance.
· The 72h-EC10 for yield inhibition (EYC10) was 6.5 mg/L with a 95% confidence interval ranging from 5.8 to 7.2 mg/L.
· The 72h-EC50 for yield inhibition (EYC50) was 18 mg/L with a 95% confidence interval ranging from 17 to 18 mg/L.
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