Methyltrioctylammonium chloride

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Endpoint:

toxicity to microorganisms, other

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Data is from peer reviewed journal

Justification for type of information:

Data is from peer reviewed journal

Qualifier:

according to guideline

Guideline:

other: as mentioned below

Principles of method if other than guideline:

Bioassay for the growth inhibition of microorganism Cellulomorias sp. was performed

GLP compliance:

not specified

Specific details on test material used for the study:

- Name of test material (IUPAC name): Methyltrioctylammonium chloride
- Molecular formula: C25H54ClN
- Molecular weight: 404.162 g/mole
- Smiles :[N+](CCCCCCCC)(CCCCCCCC)(CCCCCCCC)C.[ClH-]
- Inchl: 1S/C25H54N.ClH/c1-5-8-11-14-17-20-23-26(4,24-21-18-15-12-9-6-2)25-22-19-16-13-10-7-3;/h5-25H2,1-4H3;1H/q+1;/p-1
- Substance type: Organic
- Physical state: Viscous liquid (colorless to yellow)

Analytical monitoring:

not specified

Vehicle:

not specified

Test organisms (species):

other: Cellulomorias sp.

Details on inoculum:

- Strain: 21399
- Laboratory culture: ATCC (American Type Culture Collection)
- Preparation of inoculum for exposure: Inoculums were prepared from 100 cm3 cultures grown in 250 cm3 Erlenmeyer flasks on a rotary shaker.
- Initial biomass concentration: 107 cells cm-3.
-Test organism were inoculated in a synthetic medium prepaired by adding glucose 6 mM; (NH4)2HP04 4 mM; K2HPO4 6 mM; KH2P04 10 mM; KCI 7 mM; EDTA 1 mM; vitamins and trace elements.

Test type:

static

Water media type:

other: synthetic medium

Total exposure duration:

17 h

Test temperature:

26.85 deg C

pH:

6.8

Details on test conditions:

- Test vessel: 100 cm3 Erlenmeyer flasks equipped with a side tube.
- fill volume: 50 cm3
- Incubation was made on a rotary shaker, 120 rev min-1.
- Growth was measured turbid metrically at 610 nm

Reference substance (positive control):

not specified

Key result

Duration:

17 h

Dose descriptor:

EC50

Effect conc.:

> 0.1 - < 0.2 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: biomass

Duration:

17 h

Dose descriptor:

EC50

Effect conc.:

> 0.2 - < 0.4 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: Exponential growth rate

Conclusions:

The Effect concentration (EC50) of test material Methyltrioctylammonium chloride on Cellulomonas sp. was observed to be in the range 0.2 – 0.4 mg/l for the exponential growth rate and the effect concentration (EC50) on biomass was observed to be in the range 0.1 – 0.2.

Executive summary:

Bioassay for the inhibition of growth of Cellulomonas sp. was performed for the test material Methyltrioctylammonium chloride.Test organism were inoculated in a synthetic medium prepaired by adding glucose 6 mM; (NH4)2HP04 4 mM; K2HPO4 6 mM; KH2P04 10 mM; KCI 7 mM; EDTA 1 mM; vitamins and trace elements. Inoculums were prepared from 100 cm³cultures grown in 250 cm³Erlenmeyer flasks on a rotary shaker.

Initial cell concentration was recorded to be 10⁷cells cm^-3.The experiments were performed in 100cm³Erlenmeyer flasks equipped with a side tube, making direct measurements possible. The flasks contained 50cm³medium. Incubation was made on a rotary shaker, 120 rev min^-1. Growth was measured turbid metrically at 610 nm.The exponential growth rate was determined from the linear part of the growth curves by means of linear regression analysis.

 

The Effect concentration (EC50) of test material Methyltrioctylammonium chloride on Cellulomonas sp. was observed to be in the range 0.2 – 0.4 mg/l for the exponential growth rate and the effect concentration (EC50) on biomass was observed to be in the range 0.1 – 0.2 mg/l. Based on the above effect concentration test material can be considered toxic to microorganisms.

Description of key information

Toxicity to microorganism:

Bioassay for the inhibition of growth of Cellulomonas sp. was performed for the test material Methyltrioctylammonium chloride.Test organism were inoculated in a synthetic medium prepaired by adding glucose 6 mM; (NH4)2HP04 4 mM; K2HPO4 6 mM; KH2P04 10 mM; KCI 7 mM; EDTA 1 mM; vitamins and trace elements. Inoculums were prepared from 100 cm³cultures grown in 250 cm³Erlenmeyer flasks on a rotary shaker.

Initial cell concentration was recorded to be 10⁷cells cm^-3.The experiments were performed in 100cm³Erlenmeyer flasks equipped with a side tube, making direct measurements possible. The flasks contained 50cm³medium. Incubation was made on a rotary shaker, 120 rev min^-1. Growth was measured turbid metrically at 610 nm.The exponential growth rate was determined from the linear part of the growth curves by means of linear regression analysis.

 

The Effect concentration (EC50) of test material Methyltrioctylammonium chloride on Cellulomonas sp. was observed to be in the range 0.2 – 0.4 mg/l for the exponential growth rate and the effect concentration (EC50) on biomass was observed to be in the range 0.1 – 0.2 mg/l.Based on the above effect concentration test material can be considered toxic to microorganisms.

Key value for chemical safety assessment

EC50 for microorganisms:

0.2 mg/L

Additional information

Toxicity to microorganism:

1. Bioassay for the inhibition of growth of Cellulomonas sp. was performed for the test material Methyltrioctylammonium chloride.Test organism were inoculated in a synthetic medium prepaired by adding glucose 6 mM; (NH4)2HP04 4 mM; K2HPO4 6 mM; KH2P04 10 mM; KCI 7 mM; EDTA 1 mM; vitamins and trace elements. Inoculums were prepared from 100 cm³cultures grown in 250 cm³Erlenmeyer flasks on a rotary shaker.

Initial cell concentration was recorded to be 10⁷cells cm^-3.The experiments were performed in 100cm³Erlenmeyer flasks equipped with a side tube, making direct measurements possible. The flasks contained 50cm³medium. Incubation was made on a rotary shaker, 120 rev min^-1. Growth was measured turbid metrically at 610 nm.The exponential growth rate was determined from the linear part of the growth curves by means of linear regression analysis.

 

The Effect concentration (EC50) of test material Methyltrioctylammonium chloride on Cellulomonas sp. was observed to be in the range 0.2 – 0.4 mg/l for the exponential growth rate and the effect concentration (EC50) on biomass was observed to be in the range 0.1 – 0.2 mg/l.Based on the above effect concentration test material can be considered toxic to microorganisms.

2.Bioassay for the inhibition of growth of Sporocytophaga myxococcoides was performed for the test material Methyltrioctylammonium chloride.Test organism were inoculated in a synthetic medium prepaired by adding glucose 6 mM; (NH4)2HP04 4 mM; K2HPO4 6 mM; KH2P04 10 mM; KCI 7 mM; EDTA 1 mM; vitamins and trace elements. Inoculums were prepared from 100 cm³cultures grown in 250 cm³Erlenmeyer flasks on a rotary shaker.

Initial cell concentration was recorded to be 10⁷cells cm^-3.The experiments were performed in 100cm³Erlenmeyer flasks equipped with a side tube, making direct measurements possible. The flasks contained 50cm³medium. Incubation was made on a rotary shaker, 120 rev min^-1. Growth was measured turbid metrically at 610 nm.The exponential growth rate was determined from the linear part of the growth curves by means of linear regression analysis.

The Effect concentration (EC50) of test material Methyltrioctylammonium chloride onSporocytophaga myxococcoides was observed to be in the range 0.8 - 0.16 mg/l for the exponential growth rate and the effect concentration (EC50) on biomass was observed to be in the range 0.4 – 0.8 mg/l.Based on the above effect concentration test material can be considered toxic to microorganisms.