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EC number: 820-015-3 | CAS number: 129874-15-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- GLP compliance:
- yes
- Vehicle:
- yes
- Test organisms (species):
- Oncorhynchus mykiss (previous name: Salmo gairdneri)
- Details on test organisms:
- Acclimation period: 20.06-15.07-1996
Acclimation conditions: temperature: 9.0-19.5˚C. Dissolved oxygen: > 80% air saturation value
At the end of the test a sample of 10 trout was weighed and measured. Mean length: 48.9 mm; mean weight: 1.10 g
The sizes of the animals were found to be in approximately normal Gaussian distribution. - Test type:
- not specified
- Water media type:
- freshwater
- Total exposure duration:
- 96 h
- Hardness:
- 286 mg CaCO3/L
- Test temperature:
- Range of temperature readings: 15.1-17.5˚C
- pH:
- Range of pH values: 8.0-8.8
- Dissolved oxygen:
- Range of dissolved oxygen values: 90-96% (air saturation value)
- Reference substance (positive control):
- yes
- Duration:
- 24 h
- Dose descriptor:
- LC50
- Effect conc.:
- 59 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 46-75
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- 30 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 18-49
- Duration:
- 72 h
- Dose descriptor:
- LC50
- Effect conc.:
- 16 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 12-22
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 13 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 10-18
- Sublethal observations / clinical signs:
Cumulative percent mortalities, test species: Oncorhynchus mykiss
Concentration of test material (mg/l)
Control
Exposure period (hr)
10
18
32
56
100
0
24
0
0
0
28.6
100
0
48
0
57.1
57.1
57.1
100
0
72
14.6
100
85.7
100
100
0
96
42.9
100
85.7
100
100
0
- Validity criteria fulfilled:
- yes
- Conclusions:
- The estimated LC50 values of 59 mg/l at 24 hrs and 13 mg/l at 96 hrs support these observations.
Reference
Description of key information
LC50 values is 13 mg/l at 96 hrs.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 13 mg/L
Additional information
This report contains a study to investigate the acute toxicity of BENZANIL RUBINE 2BL (Direct Red 83-1) to rainbow trout (oncorhynchus mykiss). The objective of this study was to determine the 96 hr median lethal concentration (LC50). 5 concentrations were tested 10, 18, 32, 56 and 100 mg/l.
The results of this study indicate that at concentration above 18 mg/l is fairly rapidly lethal to rainbow trout. The estimated median period of survival, of the exposed fish, obtained for at levels of 56, 32 and 18 mg/l, were 39hr, 57hr and 47hr respectively. The lowest test concentration which caused 100% mortality of exposure fish within 96 was 18 mg/l. The highest no-observed lethal effect concentration of the test substance to rainbow trout could not be determined as there was 42.9% mortality at the lowest test concentration.
Dilution of the sample from 18 mg/l to 10 mg/l significantly increases the survival time of the trout. The estimated LC50 values of 59 mg/l at 24 hrs and 13 mg/l at 96 hrs support these observations.
A mortality of 0% was observed in the control tank at the end of the test period. This is within the range of mortality acceptable for validation of the test results.
The temperature of the control and test tanks was slightly above the recommended limit towards the end of the test, but as there was no pattern of increase in temperature, this was not thought to have adversely affected the data.
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