Cuprate(4-), [μ-[[7,7'-[[6-[(2-hydroxyethyl)amino]-1,3,5-triazine-2,4-diyl]diimino]bis[4-(hydroxy-κO)-3-[[2-(hydroxy-κO)-5-sulfophenyl]azo-κN1]-2-naphthalenesulfonato]](8-)]]di-, tetrasodium

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

28 days

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)

GLP compliance:

yes

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, adapted

Details on inoculum:

- Source of inoculum/activated sludge: Dihua sewage treatment plant, Taiwan

Duration of test (contact time):

29 d

Initial conc.:

1 g/L

Based on:

test mat.

Parameter followed for biodegradation estimation:

CO2 evolution

Reference substance:

acetic acid, sodium salt

Remarks:

Sodium acetate

Parameter:

% degradation (CO2 evolution)

Value:

ca. 0

Sampling time:

29 d

Results with reference substance:

At Day 14 during the study, the biodegradation was 73.92 %.

At the end of the test, the CO2 production of blank control groups were 41.08 mg/L.

Table 1. The percentage biodegradation of the test groups

Day	Percentage of biodegradation (% ThCO2)^a
	Test group
	Flask 1	Flask 2	Mean	Difference of replicate^b
2	-0.41	1.03	0.31	-1.45
5	0.00	1.03	0.52	-1.03
7	0.52	3.00	1.76	-2.48
9	1.03	2.69	1.86	-1.66
14	-0.31	1.14	0.41	-1.45
19	-0.83	-0.83	-0.83	0.00
23	0.21	-1.24	-0.52	1.45
27	1.03	-2.28	-0.62	3.31
29	0.41	-2.48	-1.03	2.90

^a Percentage of biodegradation (% ThCO₂) = (cumulative CO₂ of test group (mg) -cumulative CO₂ of blank control group (mg)) / (added TOC (mg) × 3.67) ×100%

^b Difference of replicate = Flask 1- Flash 2

 

Table 2. The percentage biodegradation of the reference and toxicity control group

Day	Percentage of biodegradation (% ThCO2)^a
	Referencecontrol group	Toxicity control group
	Flask 5	Flask 6
2	20.48	4.73
5	45.23	16.28
7	60.05	25.16
9	68.05	29.63
14	73.92	31.36
19	76.80	31.94
23	79.04	32.36
27	79.89	32.25
29	79.89	31.94

^a Percentage of biodegradation (% ThCO₂) = (cumulative CO₂ of reference control group or toxicity control (mg) - cumulative CO₂ of toxicity control group (mg)) / (added TOC (mg) × 3.67) ×100%

Validity criteria fulfilled:

yes

Interpretation of results:

not readily biodegradable

Conclusions:

According to OECD 301B test method, Everdirect SH12 was not be considered readily biodegradable.

Executive summary:

This test using the procedures outlined in the SuperLab Study Plan for M62-151100090001EN which is based on the SOP for the OECD 301B (SOPE-503) and OECD 301B (OECD, 1992). The results of this OECD 301B test for Everdirect SH12 show that all validity criteria were met. Everdirect SH12 was not be considered readily biodegradable under the test conditions and the percentage biodegradation of Everdirect SH12 in sludge was less than 60 %, around 0%.

Description of key information

Not readily biodegradable (OECD TG 301B).

No significant biodegradation was noted.

Key value for chemical safety assessment

Biodegradation in water:

under test conditions no biodegradation observed

Additional information

The total amount of evolved CO2 in the blank control group was 41.08 mg/L. The difference between replicates of test group was less than 20%. At Day 14 during the study, the biodegradation of the reference control article was 73.92 % and the percent biodegradation in the toxicity control was 31.36 %. All test validity criteria were met. Everdirect SH12 was not be considered readily biodegradable under the test conditions and the percentage biodegradation of Everdirect SH12 in sludge was less than 60 %, around 0%

.